Differential recognition of histone H10 by monoclonal antibodies during cell differentiation and the arrest of cell proliferation.

The Journal of Biological Chemistry
C GorkaS Khochbin

Abstract

Individual anti-H1(0) monoclonal antibodies were screened in an immunolocalization assay to isolate clones able to recognize H1(0) in a differentiation-dependent manner using a murine erythroleukemia cell line. Two clones were selected, one recognizing H1(0) only in differentiating cells (clone 27 antibody), and the other recognizing the protein constitutively (clone 34 antibody). Both antibodies recognized a restricted region of the protein located at the N-terminal part of the globular domain. Amino acids 24-30, essential for the recognition of the protein by the clone 27 antibody, are extremely conserved in all known H1(0)-like proteins from sea urchin to human. Within these residues, proline 26, responsible for a bend in this region, plays a particularly important role in the epitope recognition. The region involved in the protein recognition by clone 34 antibody is larger and encompasses amino acids 20-30. However, proline 26 does not play an essential role in the structure of this epitope. Detailed analysis of the differential recognition of H1(0) in chromatin during cell differentiation and proliferation suggests that the modification of chromatin structure as well as that of H1(0) conformation can account for this effec...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·P A Marks, R A Rifkind
Dec 3, 1990·Molecular and Cellular Biochemistry·T KawaiT Satake
Jan 18, 1990·Molecular and Cellular Biochemistry·J S Zlatanova
Jun 1, 1989·European Journal of Immunology·S DoussonJ J Lawrence
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T LieberG Childs
Feb 11, 1987·Journal of Immunological Methods·M M McKinney, A Parkinson
Dec 25, 1980·Nature·J AllanF X Aviles
Feb 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D PrussA P Wolffe
Jun 1, 1995·Experimental Cell Research·D GrunwaldS Khochbin
Oct 15, 1994·European Journal of Biochemistry·S Khochbin, A P Wolffe
Dec 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Zlatanova, D Doenecke
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D KrylovJ Zlatanova
Jan 1, 1996·Nucleic Acids Research·A D Baxevanis, D Landsman
Mar 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A P WolffeS Dimitrov

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Citations

Feb 28, 2003·Biology of Reproduction·Germaine FuHugh J Clarke
Nov 17, 2001·In Vitro Cellular & Developmental Biology. Animal·K Uehara, J Thelu
Jul 12, 2005·Biological Chemistry·Nicole HappelDetlef Doenecke
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Magdalena MedrzyckiYuhong Fan
Oct 4, 2003·Microbiology and Immunology·Yoshiyuki Kanai
Apr 5, 2000·The Journal of Biological Chemistry·C LemercierS Khochbin
Feb 27, 2004·The Journal of Peptide Research : Official Journal of the American Peptide Society·K FoulonJ Rochette

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