Differential regulation and substrate preferences in two peptide transporters of Saccharomyces cerevisiae

Eukaryotic Cell
Houjian CaiJeffrey M Becker

Abstract

Dal5p has been shown previously to act as an allantoate/ureidosuccinate permease and to play a role in the utilization of certain dipeptides as a nitrogen source in Saccharomyces cerevisiae. Here, we provide direct evidence that dipeptides are transported by Dal5p, although the affinity of Dal5p for allantoate and ureidosuccinate is higher than that for dipeptides. Allantoate, ureidosuccinate, and to a lesser extent allantoin competed with dipeptide transport by reducing the toxicity of the peptide Ala-Eth and decreasing the accumulation of [(14)C]Gly-Leu. In contrast to the well-studied di/tripeptide transporter Ptr2p, whose substrate specificity is very broad, Dal5p preferred to transport non-N-end rule dipeptides. S. cerevisiae W303 was sensitive to the toxic peptide Ala-Eth (non-N-end rule peptide) but not Leu-Eth (N-end rule peptide). Non-N-end rule dipeptides showed better competition with the uptake of [(14)C]Gly-Leu than N-end rule dipeptides. Similar to the regulation of PTR2, DAL5 expression was influenced by the addition of Leu and by the CUP9 gene. However, DAL5 expression was downregulated in the presence of leucine and the absence of CUP9, whereas PTR2 was upregulated. Toxic dipeptide and uptake assays indicated t...Continue Reading

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Citations

Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Cheol-Sang Hwang, Alexander Varshavsky
Feb 5, 2013·Eukaryotic Cell·Nico DunkelJoachim Morschhäuser
Mar 16, 2012·Genetics·Per O Ljungdahl, Bertrand Daignan-Fornier
Jun 3, 2011·Protein Science : a Publication of the Protein Society·Alexander Varshavsky
Nov 19, 2013·Environmental Microbiology·Laura TreuStefano Campanaro
Aug 15, 2015·Microbial Cell Factories·Robert H BischofBernhard Seiboth
Mar 10, 2015·Molecular Biology and Evolution·Souhir MarsitVirginie Galeote
Nov 12, 2014·Molecular and Cellular Biology·Shyamasree LaskarSunanda Bhattacharyya
Aug 6, 2016·Mechanisms of Ageing and Development·Sara VerbandtKarin Thevissen
Apr 19, 2018·IUBMB Life·Anand Kumar Bachhawat, Shambhu Yadav
Jun 3, 2017·Bio-protocol·Houjian CaiJeffrey M Becker
Nov 7, 2017·Bio-protocol·Melinda HauserJeffrey M Becker
Mar 6, 2012·Nature Chemical Biology·Marta Rubio-TexeiraJohan M Thevelein
Feb 16, 2018·Bioscience, Biotechnology, and Biochemistry·Kenji Kitamura, Eldaa Zefany Banami Kinsui
Jun 27, 2020·Frontiers in Genetics·Carmen Becerra-RodríguezVirginie Galeote

Related Concepts

Ureidosuccinic acid, zinc (1:1) salt, (L)-isomer
CUP9 protein, S cerevisiae
PTR2 protein, S cerevisiae
DAL5 protein, S cerevisiae
Woun'dres
Aspartic Acid, Magnesium-Potassium (2:1:2) Salt
Dipeptides
Leucine
Saccharomyces cerevisiae
Substrate Specificity

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