Abstract
Agents that increase intracellular cAMP are frequently growth inhibitory for lymphocytes and induce apoptosis in cortical thymocytes by regulating gene expression. In the present study, immediate early gene expression was examined in WEHI7.2 thymoma cells undergoing cAMP-mediated apoptosis. Temporal differences in c-fos, junB, and inducible cAMP early repressor (ICER) steady-state mRNA levels were observed after forskolin exposure. Maximal induction of c-fos and junB occurred within 1 h, returning to basal levels by 3.5 h. In contrast, a 1.5-h time lag was observed before ICER transcript levels increased, reaching maximal levels after 3.5 h. This rise in expression, correlating with the decrease in c-fos and junB levels, preceded apoptotic DNA fragmentation by 1.5 h. Transient expression of ICER promoter constructs demonstrated that cAMP responsiveness occurred through cAMP-autoregulatory response element (CARE)3/4, two of the four proposed response elements in the ICER promoter. In contrast to the cAMP-responsive cell line JEG-3, CARE1/2 was not functional for cAMP-activated transcription in WEHI7.2 cells. An observed differential binding pattern of WEHI and JEG nuclear extracts to these elements may account for the cell-speci...Continue Reading
Citations
Feb 15, 2001·The Biochemical Journal·H ThonbergB Cannon
Jul 18, 2009·The Journal of Biological Chemistry·Liheng ShiGladys Y-P Ko
Jan 9, 2008·The Journal of Biological Chemistry·Yinghui HuShelley J Russek
Mar 17, 2004·Molecular and Cellular Biology·Akari InadaYutaka Seino
Apr 15, 2004·Molecular and Cellular Biology·Ghia EuskirchenMichael Snyder
Jul 12, 2003·Circulation Research·Mark A Sussman
Oct 18, 2018·Journal of Cancer Research and Clinical Oncology·Qiang LiWei-Xi Shen
Mar 7, 2007·Journal of Cellular Biochemistry·Kristine MisundLiv Thommesen
Jan 10, 2004·Journal of Neurochemistry·Barbara MioduszewskaLeszek Kaczmarek
Sep 22, 2005·Journal of Neuroscience Research·Corinne M SpencerThomas A Houpt
Aug 27, 2005·Endocrinology·Martina FinkDamjana Rozman
Jul 16, 1999·Molecular Endocrinology·D A KruegerD R Dowd
Oct 19, 1999·Molecular and Cellular Biology·M UbedaJ F Habener
Mar 1, 2003·The Journal of Biological Chemistry·James R MeadDipak P Ramji
May 9, 2000·The Journal of Biological Chemistry·D A KruegerD R Dowd
Feb 8, 2000·The Journal of Biological Chemistry·G ZauliM Vitale