Differential regulation of antigen-specific IgG4 and IgE antibodies in response to recombinant filarial proteins

International Immunology
O GarraudT B Nutman

Abstract

Having identified two recombinant filarial proteins (Ov27 and OvD5B) that induced patient peripheral blood mononuclear cells to produce antigen-specific IgG4/IgE antibodies in vitro, we assessed the role these filarial antigens play in inducing antigen-specific isotype switching (gamma 4 and epsilon) in the absence of T cells. Purified CD19+ s gamma-/s epsilon- B cells were cultured with either of these antigens in the presence of anti-CD40 mAb and human IL-4. Both antigen and polyclonal signals delivered by IL-4 (or IL-13) were necessary for the induction of specific IgG4/IgE antibodies. To assess the role played by cytokines produced by B lymphocytes in antigen-driven selection of the gamma 4 or epsilon isotype, neutralizing anti-cytokine antibodies were used in vitro. While anti-IL-12 antibodies did not alter the antigen-specific IgG4/IgE production, anti-IL-6, anti-IL-13 and anti-tumor necrosis factor-alpha antibodies significantly inhibited the production of IgG4/IgE. Anti-IL-2 and anti-IL-10 antibodies appeared to down-regulate antigen-specific IgG4 antibodies without affecting antigen-specific IgE antibodies. Although anti-CD21 antibodies had no effect on specific IgE antibodies, they up-regulated specific IgG4 antibodie...Continue Reading

Citations

Jul 12, 2003·Trends in Parasitology·Olivier GarraudThomas B Nutman
May 22, 2001·Clinical and Experimental Immunology·M C MohantyB Ravindran
Feb 9, 1999·The Journal of Allergy and Clinical Immunology·O GarraudT B Nutman
Oct 9, 2001·Parasite Immunology·H FaulknerJ E Bradley
Aug 29, 2020·Acta Tropica·Norbert W BrattigRolf Garms

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