Differential Regulation of Bladder Pain and Voiding Function by Sensory Afferent Populations Revealed by Selective Optogenetic Activation

Frontiers in Integrative Neuroscience
Jennifer J DeBerryRobert W Gereau

Abstract

Bladder-innervating primary sensory neurons mediate reflex-driven bladder function under normal conditions, and contribute to debilitating bladder pain and/or overactivity in pathological states. The goal of this study was to examine the respective roles of defined subtypes of afferent neurons in bladder sensation and function in vivo via direct optogenetic activation. To accomplish this goal, we generated transgenic lines that express a Channelrhodopsin-2-eYFP fusion protein (ChR2-eYFP) in two distinct populations of sensory neurons: TRPV1-lineage neurons (Trpv1Cre;Ai32, the majority of nociceptors) and Nav1.8+ neurons (Scn10aCre;Ai32, nociceptors and some mechanosensitive fibers). In spinal cord, eYFP+ fibers in Trpv1Cre;Ai32 mice were observed predominantly in dorsal horn (DH) laminae I-II, while in Scn10aCre;Ai32 mice they extended throughout the DH, including a dense projection to lamina X. Fiber density correlated with number of retrogradely-labeled eYFP+ dorsal root ganglion neurons (82.2% Scn10aCre;Ai32 vs. 62% Trpv1Cre;Ai32) and degree of DH excitatory synaptic transmission. Photostimulation of peripheral afferent terminals significantly increased visceromotor responses to noxious bladder distension (30-50 mmHg) in bot...Continue Reading

References

Jan 22, 1992·The Journal of Comparative Neurology·G Battaglia, A Rustioni
Aug 1, 1989·The American Journal of Physiology·B MalloryW C De Groat
Jun 1, 1994·Journal of Neurophysiology·J N Sengupta, G F Gebhart
Jul 1, 1993·The American Journal of Physiology·C L ChengW C de Groat
Aug 1, 1993·The American Journal of Physiology·L A Birder, W C de Groat
Dec 31, 1996·Brain Research. Molecular Brain Research·J A BlackS G Waxman
Jul 18, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M A Vizzard
Oct 12, 2000·Journal of Neurophysiology·V K SheaE R Perl
Aug 6, 2002·Nature Neuroscience·L A BirderM J Caterina
Mar 30, 2004·Genesis : the Journal of Genetics and Development·Nitin AgarwalRohini Kuner
Aug 24, 2005·Nature Neuroscience·Edward S BoydenKarl Deisseroth
Dec 2, 2005·The Journal of Comparative Neurology·Julie A ChristiansonBrian M Davis
Oct 20, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Naoki YoshimuraSatoshi Seki
May 19, 2007·Digestive Diseases and Sciences·Fang ZhongKlaus Bielefeldt
Jul 14, 2007·The Journal of Physiology·D DalyD Grundy
Apr 18, 2008·The Journal of Comparative Neurology·Kenneth E FasanellaBrian M Davis
Jan 23, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sacha A MalinBrian M Davis
May 20, 2009·Proceedings of the National Academy of Sciences of the United States of America·Daniel J CavanaughDavid J Anderson
Oct 23, 2009·The Journal of Urology·Ana CharruaAntónio Avelino
Dec 3, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Herbert E CovingtonEric J Nestler
Dec 9, 2010·The EMBO Journal·Santosh K MishraMark A Hoon
Apr 1, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel J CavanaughAllan I Basbaum
Jul 15, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel J CavanaughAllan I Basbaum
Mar 28, 2012·Molecular Pain·Lara W CrockRobert W Gereau

❮ Previous
Next ❯

Citations

Feb 15, 2019·Nature Reviews. Urology·Warren M Grill, Jennifer J DeBerry
Jul 1, 2020·Frontiers in Neural Circuits·Erika K HardingRobert P Bonin
Feb 20, 2020·Critical Reviews in Biochemistry and Molecular Biology·Xiang XuXiaofeng Jia
Aug 17, 2018·Pain·Aaron D Mickle, Robert W Gereau
May 29, 2018·Molecular Neurobiology·Aryo ZareGommert A van Koeveringe
Feb 5, 2021·Nature Reviews. Urology·Matthias VannesteWouter Everaerts
Aug 11, 2021·Neuromodulation : Journal of the International Neuromodulation Society·Zhonghan Zhou, Limin Liao
Nov 18, 2021·Investigative Ophthalmology & Visual Science·Neal E MecumIan D Meng

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
antisense oligodeoxynucleotide

Software Mentioned

Nikon Elements
Spike2
Igor Pro
Adobe Photoshop
Patchmaster

Related Concepts

Related Feeds

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.