PMID: 7537557Dec 1, 1994Paper

Differential regulation of CD43 glycoforms on CD4+ and CD8+ T lymphocytes in graft-versus-host disease

Glycobiology
L G ElliesH J Ziltener

Abstract

Two distinct T-cell glycoforms of CD43 result from differential glycosylation of a single gene product in vivo. The 115 kDa glycoform carries mainly tetrasaccharides and is a pan T-cell marker, whereas the 130 kDa glycoform carries mainly hexasaccharides and is associated with T-cell activation. CD43 has been shown to play a role both in enhancing and inhibiting cell adhesion; however, the function of the individual glycoforms is unknown. We have examined the distribution and regulation of the CD43 glycoforms in a murine model of acute graft-versus-host disease (GVHD) using monoclonal antibodies (mAbs) S7 and 1B11 specific for the 115 and 130 kDa CD43 glycoforms, respectively. An increase in T-lymphocyte CD43 130 kDa expression occurred during GVHD from day 4 onwards and coincided with splenomegaly and upregulation of the beta 1-6GlcNAc transferase (C2GnT), the key enzyme responsible for the addition of complex O-glycan branching to CD43. When T-lymphocyte subsets were examined for CD43 expression, we found that in GVHD, both CD43 glycoforms were upregulated on CD4+ T cells. However, in CD8+ T cells, CD43 115 kDa was downregulated while CD43 130 kDa was dramatically upregulated, such that two distinct CD8+1B11+ T-cell subsets w...Continue Reading

Citations

Dec 2, 1999·Immunologic Research·Y RosensteinG Pedraza-Alva
Jul 9, 2008·Immunology and Cell Biology·L A Earl, L G Baum
May 28, 2005·Journal of Biomolecular Structure & Dynamics·Kasinadar Veluraja, Claudio J Margulis
Sep 29, 2004·Biological Chemistry·Paul ZiprinAra W Darzi
Jun 19, 2004·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Jeff S FriedmanSteven J Burakoff
Jul 11, 2006·Immunological Reviews·Hirokazu HikonoDavid L Woodland
Sep 28, 2002·Nature Immunology·Mark A DanielsStephen C Jameson
Oct 12, 2000·The Journal of Biological Chemistry·G Pedraza-AlvaY Rosenstein
Feb 16, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eric PerkeyIvan Maillard
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