Differential regulation of CDX1 and CDX2 gene expression by deficiency in methyl group donors

Biochimie
Xiaohong LuFarès Namour

Abstract

The CDX2 and CDX1 homeobox genes have respectively a tumour suppressor and proliferative role in the intestinal epithelium. We analyzed DNA methylation and histones modifications associated with CDX2 and CDX1 promoters in two human colon cancer cell lines expressing differentially these genes, Caco2/TC7 [CDX2 positive-CDX1 negative] and HT29 [CDX2 negative-CDX1 negative] cells. Chromatin immunoprecipitation experiments indicated that CDX2 and CDX1 gene expression correlated with a histone modifications pattern characterizing active chromatin (H3K4 trimethylated and H3 acetylated). Bisulfite DNA sequencing and methylation-specific PCR showed that CDX2 and CDX1 promoters display no methylation in HT29 cells even though both genes are not expressed. In contrast, the CDX1 promoter is methylated in Caco2/TC7. DNA demethylation by 5aza-dC or the combination of 5aza-dC plus SAHA, an inhibitor of histone deacetylases, restored CDX1 expression in Caco2/TC7 cells but these treatments were inefficient on both CDX2 and CDX1 in HT29 cells. Thus, in colon cancer cells the changes in chromatin conformation are heterogeneous and repression of CDX2 and CDX1 in HT29 cells is not due to epigenetic mechanisms. In vivo, dietary deprivation of methy...Continue Reading

References

Feb 1, 1996·Molecular and Cellular Biology·E Suh, P G Traber
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·F BeckJ B Furness
Dec 2, 1999·Gastroenterology·P SoubeyranJ L Iovanna
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 19, 2002·The Journal of Biological Chemistry·Eun Ran SuhPeter G Traber
Aug 30, 2003·The Journal of Biological Chemistry·Takao HinoiEric R Fearon
Sep 13, 2003·Oncogene·Claire Domon-DellJean-Noël Freund
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·N A C S WongW F Bodmer
Feb 24, 2004·American Journal of Epidemiology·Linda Sharp, Julian Little
Jun 19, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Vassil KaimaktchievChristopher L Corless
Oct 23, 2004·International Journal of Cancer. Journal International Du Cancer·Miguel A SanjoaquinTimothy J Key
May 17, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Loïc Le MarchandBrian E Henderson
Apr 28, 2006·Gut·B Van GuelpenR Palmqvist
Jan 27, 2007·The American Journal of Pathology·Fairouz BenahmedIsabelle Duluc
May 31, 2007·International Journal of Cancer. Journal International Du Cancer·MingZhou GuoJames G Herman

❮ Previous
Next ❯

Citations

Dec 17, 2008·The British Journal of Nutrition·Sophie TesseraudIban Seiliez
Jul 17, 2012·The British Journal of Nutrition·Aude BressenotLaurent Peyrin-Biroulet
Feb 23, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Claudio D'AddarioA Carlo Altamura
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michael P VerziRamesh A Shivdasani
Feb 11, 2014·Nutrition Reviews·Jean-Paul Lallès
Feb 1, 2015·Medical Oncology·Ali Akbar Samadani, Haleh Akhavan-Niaki
Aug 19, 2009·Cellular Signalling·Felix KruegerStephen J Gaunt
Dec 14, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Vipen Batra, Thomas Paul Asir Devasagayam
Jul 12, 2014·Journal of Affective Disorders·Bernardo Dell'OssoA Carlo Altamura
Aug 23, 2020·Cells·Sepideh Fallah, Jean-François Beaulieu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.