Differential regulation of cellular maturation in chondrocytes and osteoblasts by glycine

Cell and Tissue Research
Yoshifumi TakahataYukio Yoneda

Abstract

Previous studies have demonstrated the functional expression, by osteoblasts, of N-methyl-D-aspartate (NMDA) receptors responsible for the promotion of cellular differentiation in bone. We have now evaluated the possible role of the endogenous co-agonist of NMDA receptors, glycine (Gly), in chondrogenesis. In ex vivo organotypic cultures of fetal mouse tibias, proximal and distal cartilaginous primordia were significantly increased in the presence of Gly, with the osteogenic center being unchanged. Exposure to Gly drastically increased mRNA expression of the calcified chondrocyte marker osteopontin, without markedly affecting that of a proliferating chondrocyte marker or a hypertrophic chondrocyte marker, as shown in organotypic cultures by in situ hybridization analysis. Gly significantly increased Ca2+ accumulation, osteopontin mRNA expression, and alkaline phosphatase activity in cultured rat costal chondrocytes, without significantly affecting those in cultured rat calvarial osteoblasts. The increase induced by Gly was significantly prevented by an NMDA receptor channel blocker and an antagonist at the Gly site on NMDA receptors, but not by an inhibitory Gly receptor antagonist or a Gly transporter inhibitor, in cultured ch...Continue Reading

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Citations

Oct 6, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takeshi Takarada
Jul 3, 2013·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takeshi Takarada
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Jan 19, 2012·Journal of Cellular Physiology·Takeshi TakaradaYukio Yoneda
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Dec 18, 2019·Cell Communication and Signaling : CCS·Csaba MattaRóza Zákány
Feb 17, 2018·Joint, Bone, Spine : Revue Du Rhumatisme·Ali MobasheriEiva Bernotiene

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