Abstract
Kinins mediate their cellular effects through B1 (B1R) and B2 (B2R) receptors, and the activation of B2R reduces collagen synthesis in cardiac fibroblasts (CF). However, the question of whether B1R and/or B2R have a role in cardiac myofibroblasts remains unanswered. CF were isolated from neonate rats and myofibroblasts were generated by an 84 h treatment with TGF-β1 (CMF). B1R was evaluated by western blot, immunocytochemistry and radioligand assay; B2R, inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), and cyclooxygenases 1 and 2 (COX-1, and COX-2) were evaluated by western blot; intracellular Ca⁺² levels were evaluated with Fluo-4AM; collagen secretion was measured in the culture media using the picrosirius red assay kit. B2R, iNOS, COX-1 and low levels of B1R but not eNOS, were detected by western blot in CF. Also, B1R, B2R, and COX-2 but not iNOS, eNOS or COX-1, were detected by western blot in CMF. By immunocytochemistry, our results showed lower intracellular B1R levels in CF and higher B1R levels in CMF, mainly localized on the cell membrane. Additionally, we found B1R only in CMF cellular membrane through radioligand displacement assay. Bradykinin (BK) B2R agonist increased intracellular ...Continue Reading
References
Feb 1, 1995·The Journal of Clinical Investigation·D J PinskyP J Cannon
Nov 22, 1997·Hypertension·H YuM P Printz
Jul 23, 1998·Hypertension·A M GallagherM P Printz
Mar 27, 1999·Journal of Molecular and Cellular Cardiology·N N KimF J Villarreal
Apr 25, 2000·Cardiovascular Research·Y Sun, K T Weber
Mar 3, 2001·Angiology·Y BalbayS Korkmaz
Jan 24, 2002·The Journal of Pharmacology and Experimental Therapeutics·Burkhard Hinz, Kay Brune
Jun 25, 2003·Circulation·Kenji HayakawaHisayoshi Fujiwara
Jul 8, 2003·The Journal of Pathology·G Gabbiani
Jul 10, 2004·Journal of Molecular and Cellular Cardiology·Takayuki SaitoAdel Giaid
Aug 20, 2004·Pharmacological Reviews·Daniel L SimmonsTimothy Hla
Mar 1, 2005·Pharmacological Reviews·L M Fredrik Leeb-LundbergBruce L Zuraw
Sep 24, 2005·Journal of Pharmacological Sciences·Marie Eve MoreauAlbert Adam
May 5, 2007·Thrombosis and Haemostasis·Nikolaos G Frangogiannis
Sep 18, 2007·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Nina ZidarDusan Stajer
May 23, 2009·Pharmacology & Therapeutics·Karen E Porter, Neil A Turner
Dec 2, 2009·Nature Reviews. Cardiology·Susanne W M van den BorneJagat Narula
Nov 26, 2010·Biochemical and Biophysical Research Communications·K RichesC Peers
Feb 8, 2011·Journal of Cardiovascular Pharmacology·Jennifer Baum, Heather S Duffy
Aug 24, 2011·Allergy·M MaurerM Magerl
Citations
Aug 16, 2014·International Journal of Cardiology·Cheng-Chih ChungYi-Jen Chen
May 14, 2016·Molecular Immunology·Pia BozaGuillermo Díaz-Araya
Jul 17, 2019·Molecular Biology Reports·Mabel CatalánGuillermo Díaz-Araya
Aug 18, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Nora HegazyAhmed Fahmy
Jan 28, 2014·Journal of Hypertension·Maria Paz OcaranzaLuis Michea
Jun 13, 2019·Biochemical and Biophysical Research Communications·Andrew Kisling, Laxmansa C Katwa