Differential regulation of G protein subunit expression in mouse oocytes, eggs, and early embryos

Developmental Biology
A E AllworthC A Ziomek

Abstract

Pertussis toxin ADP-ribosylation and Western blot analysis using G protein-specific antibodies were used to study G protein expression in mouse oocytes, eggs, and early embryos. A pertussis toxin (PT) substrate of about 40 kDa was observed in all stages, but its level was stage dependent. It decreased dramatically between germinal vesicle stage oocytes and unfertilized eggs, remained relatively constant through the early 2-cell stage, and then declined again with each cell division, reaching the lowest level at the 8- to 16-cell stage. Its level, or perhaps that of a different substrate, then increased at the blastocyst stage. Western blot analysis with antisera to the G protein alpha subunit indicated that the decrease between germinal vesicle stage oocytes and unfertilized eggs was less pronounced for the alpha subunit itself than for the PT substrate. Antisera to G protein beta subunit revealed that the difference in the amount of this subunit in germinal vesicle-stage oocytes versus unfertilized eggs was even greater than that of the PT ADP-ribosylation substrate. These results suggest that during oocyte maturation G protein beta gamma levels decline to a greater extent than alpha levels. Additional evidence supporting this...Continue Reading

References

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Citations

Mar 26, 2003·Zoological Science·Tatsuo IwasaMotoyuki Tsuda
Apr 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Maria H HamiltonJohn D Hildebrandt
Aug 1, 1997·Biochemical Pharmacology·J D Hildebrandt
Mar 15, 2008·Biochimica Et Biophysica Acta·Judith J Eckert, Tom P Fleming
Mar 9, 2004·Mechanisms of Development·Ekaterina Voronina, Gary M Wessel
Jul 1, 1992·The Journal of Experimental Zoology·S M DownsJ J Eppig
Dec 5, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·B Vanderbeld, G M Kelly

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