PMID: 11319768Apr 25, 2001Paper

Differential regulation of GDNF, neurturin, and their receptors in primary cultures of rat glial cells

Journal of Neuroscience Research
S RémyI Neveu

Abstract

Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) bind to GFR alpha-1 and GFR alpha-2 receptors, respectively, and their neurotrophic activity is mediated by the tyrosine kinase receptor, Ret. All these molecules were found to be expressed in primary cultures of rat glial cells, which were largely composed of astrocytes and maintained in serum-free medium. Although GDNF, NTN and Ret mRNA levels were at the limit of detection, RNase protection assays revealed relatively high amounts of GFR alpha-1 and GFR alpha transcripts. To characterize signals controlling their expression, glial cells were exposed to serum or treated with hormones acting through nuclear receptors and by activators of the cAMP or protein kinase C (PKC)-dependent pathways. Retinoic acid or 1,25-dihydroxyvitamin D3 appeared ineffective. In contrast, the 5-fold increase in GFR alpha-2 mRNA after 24 hr of treatment with 10(-10) M of tri-iodothyronine, suggests a physiological role of thyroid hormone in the regulation of this receptor in vivo. The serum induced a 7-fold increase in GFR alpha-1 mRNA levels. These changes may be mediated by the cAMP or PKC pathways because both forskolin and TPA up-regulated the GFR alpha-1 gene. Interestingly, ...Continue Reading

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Citations

Oct 1, 2008·Progress in Neurobiology·Ana SaavedraEmília P Duarte
Feb 19, 2013·Journal of Neurochemistry·Francesca BosciaLaura Cerchia
Aug 25, 2010·Journal of Neurochemistry·Brandon S Pruett, Michael F Salvatore
Jul 9, 2005·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Maria Pina SerraMarina Del Fiacco
Apr 23, 2013·Neurobiology of Aging·Sergey KalininDouglas L Feinstein
Jun 28, 2011·Neurobiology of Aging·Sergey KalininDouglas L Feinstein
Jan 28, 2005·Cellular and Molecular Neurobiology·Chong ZhangQingxuan Chen
Jun 9, 2009·Psychoneuroendocrinology·D W EylesT H J Burne

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