Differential regulation of genes transcribed by nucleus-encoded plastid RNA polymerase, and DNA amplification, within ribosome-deficient plastids in stable phenocopies of cereal albino mutants

Molecular Genetics and Genomics : MGG
M K Zubko, Anil Day

Abstract

We isolated stable albino plants of barley and maize by inhibiting plastid protein synthesis with streptomycin and propagating bleached seedlings in the absence of antibiotics in vitro. Albino plants are deficient in plastid translation products and plastid ribosomal RNAs, and are stable phenocopies of the barley albostrians and maize iojap mutants, which contain ribosome-free plastids. Once plastid ribosomes are lost they cannot be re-synthesized because about one-third of plastid ribosomal proteins are themselves plastid encoded. The group II/subgroup IIA intron in plastid rpl2 transcripts was not processed in albinos, providing strong evidence for the absence of plastid translation. Photosynthesis-related plastid mRNAs and plastid tRNAs were down-regulated in albino leaves. A differential influence of plastid ribosome deficiency on mRNA levels allowed us to divide genes transcribed by nucleus-encoded plastid RNA polymerase into two groups. Northern analysis revealed increases in the levels of clpP, rpl2, rpl23, rps15 and rpoB mRNAs in total RNA from albino leaves relative to those in green leaves. In contrast, albinism did not increase the band intensities of rps2 and rps4 messages. Plastid ribosome-associated factor(s) or p...Continue Reading

Citations

Feb 21, 2009·Functional & Integrative Genomics·M Muñoz-AmatriaínM P Vallés
Jan 28, 2005·Transgenic Research·Mikhajlo K ZubkotAnil Day
Apr 14, 2004·Molecular Plant-microbe Interactions : MPMI·Sanjay Kumar JainKarl-Heinz Kogel
Dec 19, 2003·Plant Physiology·Li TangMarie E Petracek
Dec 7, 2007·The Plant Cell·Maria-Elena RodioFrancesco Di Serio
Oct 11, 2005·The Plant Journal : for Cell and Molecular Biology·Vasumathi KodeAnil Day
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Pascale M Williams, Alice Barkan
Aug 9, 2003·The Plant Journal : for Cell and Molecular Biology·Elena YaronskayaBernhard Grimm
Apr 17, 2004·The Plant Journal : for Cell and Molecular Biology·Carola EmanuelThomas Börner
Sep 13, 2005·Trends in Plant Science·Wouter G van Doorn
May 29, 2004·Trends in Plant Science·David B SternAlice Barkan
May 24, 2018·Biological Reviews of the Cambridge Philosophical Society·Oleg N Tikhodeyev
Oct 21, 2017·Journal of Experimental Botany·Jian ZhangRuili Li
Apr 4, 2017·Wiley Interdisciplinary Reviews. RNA·Xiaowen ShiStephane Bentolila
Dec 1, 2019·The Science of the Total Environment·Oleg N Tikhodeyev

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