Differential regulation of glucose and glycogen metabolism in vascular smooth muscle by exogenous substrates

Journal of Molecular and Cellular Cardiology
C D Hardin, T M Roberts

Abstract

The aim of this study was to determine whether the pathways of glycolysis and glycogenolysis can be independently modulated by the provision of acetate or pyruvate as exogenous substrates. Hog carotid artery segments were allowed to replete glycogen stores to over 6 micromol/g of new 13C-labeled glycogen by incubation at 37 degrees C with 5 mM [1-13C]glucose for 6-16 h and then were isometrically contracted for 3 h with 80 mM KCl in the presence of 5 mM [2-13C]glucose and either 2 mM sodium acetate or 5 mM sodium pyruvate. Measurements were made of total lactate production, glucose utilization, glycogen utilization, isometric force, [2-13C]lactate and [3-13C]lactate production. Compared to experiments with glucose as the sole exogenous substrate, provision of pyruvate significantly decreased glucose utilization (by 28%) but insignificantly decreased glycogen utilization. In contrast, provision of acetate resulted in a statistically insignificant decrease in glucose utilization (by 23%) and an increase in glycogen utilization (by 20%). The fraction of [3-13C]pyruvate derived from glycogen that was converted to [3-13C]lactate was significantly decreased in the presence of acetate despite the enhanced glycogen utilization. Despite...Continue Reading

Citations

Jul 22, 2015·Journal of Biomedical Materials Research. Part a·Noriyuki UchidaJiro Nagatomi
Aug 17, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gerald A Dienel
Dec 31, 2002·Physiological Reviews·Giovanni E MannLuis Sobrevia
Aug 9, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Jia ShiFan He
Dec 22, 1999·The American Journal of Physiology·P G Lloyd, C D Hardin
Jun 14, 2000·American Journal of Physiology. Heart and Circulatory Physiology·T J Allen, C D Hardin
Feb 9, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·R M LeachJ T Sylvester
Feb 12, 1998·The American Journal of Physiology·C D Hardin, D R Finder
Mar 3, 1998·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·F R Bühler
Feb 7, 2001·American Journal of Physiology. Cell Physiology·P G Lloyd, C D Hardin
Dec 9, 1998·The American Journal of Physiology·T M Allen, C D Hardin

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