Differential regulation of GTPase activity by mastoparan and galparan

Archives of Biochemistry and Biophysics
M ZorkoU Langel

Abstract

The chimeric peptide galparan, composed of galanin (1-13) in the N-terminus and mastoparan in the C-terminus, was recently designed and synthesized. The effect of galparan on GTPase activity of rat brain cortical membranes was studied in comparison with the effect of mastoparan and galanin. GTPase was activated by mastoparan but it was noncompetitively inhibited by galparan, while no effect of galanin and galanin (1-13) was found in this tissue. EC50 of 12.1 +/- 2.1 microM and Hill coefficient of 2.1 +/- 0.6 was calculated for galparan from a dose-response curve and Ki of 19.1 +/- 0.3 microM was obtained by fitting the experimental data to the Michaelis-Menten equation valid in the presence of noncompetitive inhibitor. Mastoparan reversed the effect of galparan in a fully competitive manner while benzalkonium chloride did not prevent the inhibition of GTPase activity by galparan. Pertussis-toxin-catalyzed ribosylation of G proteins from rat brain cortical membranes resulted in 15% lower basal GTPase activity of our preparation but did not alter the parameters of the dose-response curve for galparan inhibition. The rate of GTP gamma S binding to G proteins from rat brain cortical membranes was not influenced by galparan. CD spec...Continue Reading

References

Dec 8, 1976·Biochimica Et Biophysica Acta·D Cassel, Z Selinger
Aug 1, 1979·Chemical & Pharmaceutical Bulletin·Y HiraiC Kitada
Jun 1, 1992·International Journal of Peptide and Protein Research·U LangelT Bartfai
Sep 1, 1991·International Journal of Peptide and Protein Research·T LandT Bartfai
Jun 14, 1991·Biochemical and Biophysical Research Communications·A TanimuraY Tojyo
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Aug 1, 1981·Analytical Biochemistry·E De VendittisV Bocchini
Apr 1, 1995·The International Journal of Biochemistry & Cell Biology·K BedecsT Bartfai
Nov 15, 1993·Biochemical and Biophysical Research Communications·M DanilenkoY Sharoni
Apr 16, 1993·Biochimica Et Biophysica Acta·T FischerM Mousli
May 9, 1997·Brain Research·S ConsoloT Bartfai
Aug 1, 1997·Biochemical Society Transactions·M MeznaJ Howl

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Citations

Feb 3, 1999·Annals of the New York Academy of Sciences·M PoogaU Langel
Feb 18, 2016·Neural Plasticity·Valerie T RamírezNibaldo C Inestrosa
Feb 14, 2002·Chemistry & Biology·Michelle FarquharJohn Howl
Aug 10, 2000·Biochimica Et Biophysica Acta·U SoometsU Langel

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