Differential regulation of human and mouse telomerase reverse transcriptase (TERT) promoter activity during testis development

Molecular Reproduction and Development
J M Weise, C Güneş

Abstract

In adult testis, telomerase activity is exclusively detectable during the early steps of spermatogenesis and is downregulated during differentiation to spermatozoa. Knowledge about telomerase activity during testis development from birth to adulthood is still scarce. Telomerase activity is regulated primarily via the transcriptional initiation of TERT expression which encodes its catalytic subunit. We used the hTERTp-lacZ transgenic mouse model that expresses the bacterial lacZ reporter gene under the control of an 8.0-kbp human TERT promoter fragment to analyze simultaneously endogenous mouse Tert gene expression as well as human TERT promoter activity during mouse testis development. We show that human TERT promoter activity increased during puberty and was highest in adult mouse testis whereas mouse Tert expression and telomerase activity were found to be high in testis from the earliest time point tested (6 days postpartum). Histological analysis revealed that beta-galactosidase expression, encoded by the lacZ reporter gene, is present in all seminiferous tubules in adult testis, but in a subset of tubules before puberty. We further analyzed the expression of SCF/ c-KIT, which was described to regulate spermatogonia prolife...Continue Reading

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Citations

May 28, 2010·Journal of Assisted Reproduction and Genetics·Kenji SatoYoshihiko Hosoi
Jul 24, 2013·Trends in Genetics : TIG·Bruno Bernardes de Jesus, Maria A Blasco
Feb 15, 2011·American Journal of Human Biology : the Official Journal of the Human Biology Council·Dan T A Eisenberg
Aug 3, 2016·Genes·Mukesh KumarÇagatay Güneş
Jan 9, 2015·Biology of Reproduction·Saffet Ozturk
Jun 19, 2019·Molecular Reproduction and Development·Priscila Ramos-IbeasAlfonso Gutiérrez-Adán
May 14, 2016·Human Molecular Genetics·Anita Cindric VranesicOtmar Huber
Dec 28, 2019·International Journal of Molecular Sciences·Yousef Ashraf Tawfik MorcosCagatay Günes
Mar 20, 2019·International Journal of Molecular Sciences·Alessandra VenturaMaria Concetta Fargnoli

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