PMID: 9538144Jun 6, 1998Paper

Differential regulation of human NK cell-associated gene expression following activation by IL-2, IFN-alpha and PMA/ionomycin

International Journal of Oncology
S MoriB Bonavida

Abstract

Natural killer (NK) cells are important in host-defense mechanisms against infection and cancer and also participate in regulation of the immune response. The functions of NK cells as well as their maturation and differentiation are regulated by various stimuli such as interleukin-2 (IL-2) and interferon-alpha (IFN-alpha). The mechanisms by which these stimuli regulate distinct NK functions are not known. This study compared the patterns of gene expression for several NK-associated genes namely perforin (PEF), granzymes A and B (GA or B), IL-1beta, IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), CD16 and NK-specific genes, NKG2A, NKG5 and NKG7 in both unstimulated and in IL-2-, IFN-alpha and PMA/Ionomycin (PMA/I)-stimulated NK cells purified from human peripheral blood. IFN-alpha enhanced mRNA expression for PEF, IFN-gamma, TNF-alpha and NKG2A, but did not affect NKG7 mRNA expression. IL-2 augmented mRNA expression for PEF, IFN-gamma, TNF-alpha, NKG2A and NKG7. PMA/I increased mRNA expression for IFN-gamma, TNF-alpha and NKG2A but did not affect mRNA expression for PEF and NKG7. Further, PMA/I inhibited the expression of CD16 mRNA. These findings demonstrate that the three NK-stimuli used share in common the regulation of s...Continue Reading

Citations

Apr 17, 2009·Clinical and Vaccine Immunology : CVI·Felix N TokaWilliam T Golde
Aug 17, 2021·Pathology Oncology Research : POR·Ning XuJianguo Li

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