Differential regulation of inducible nitric oxide synthase production in bovine and caprine macrophages

The Journal of Infectious Diseases
H AdlerT W Jungi

Abstract

Inducible nitric oxide synthase (iNOS) regulation in human and murine macrophages in vitro differs considerably. In this study, expression of macrophage iNOS in ruminants was addressed. Nitric oxide (NO) output by cattle and goat macrophages was as different as that by human and mouse macrophages. Bovine macrophages activated by heated Salmonella dublin or lipopolysaccharide (LPS) expressed high levels of iNOS mRNA, protein, and enzyme activity. Analogously cultured caprine macrophages did not respond to these and other activators by NO generation and iNOS expression. The lack of response was not due to general unresponsiveness to stimuli. Caprine iNOS mRNA was induced by stimulation of caprine macrophages with LPS, as shown by reverse transcription polymerase chain reaction. The level of mRNA expression in activated goat macrophages was lower than in resting bovine macrophages. A caprine 372-bp iNOS mRNA fragment that was sequenced closely resembled the bovine counterpart. This points to species-specific iNOS gene regulation.

Citations

Jan 27, 2004·Journal of Neuroimmunology·Michael D RothGayle C Baldwin
Feb 18, 1999·Journal of Immunological Methods·L PerlerT W Jungi
Jun 13, 1998·Research in Veterinary Science·A TipoldT W Jungi
Oct 17, 2001·Developmental and Comparative Immunology·N F NeumannM Belosevic
Apr 29, 1999·Developmental and Comparative Immunology·K J LaingC J Secombes
Jul 7, 2001·Comparative Immunology, Microbiology and Infectious Diseases·M A PanaroO Brandonisio
May 1, 1997·Veterinary Immunology and Immunopathology·J R BogdanJ A Ellis
Jun 5, 1998·Veterinary Immunology and Immunopathology·M S PampuschM P Murtaugh
Aug 7, 1999·Veterinary Immunology and Immunopathology·H SagerT W Jungi
Jun 18, 1999·Journal of Veterinary Pharmacology and Therapeutics·R P HunterA A Dees
Apr 1, 2003·Animal Health Research Reviews·Robert P Hunter
Mar 17, 2006·Critical Care Medicine·Martin WestphalDaniel L Traber
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Mar 20, 2001·American Journal of Respiratory and Critical Care Medicine·K SoejimaL Varig
May 23, 2014·Parasitology Research·Liliana M R SilvaAnja Taubert
May 6, 2008·Nitric Oxide : Biology and Chemistry·Petra ZelnickovaMartin Faldyna
Apr 25, 2002·Experimental Parasitology·Sergio H SeabraRenato A DaMatta
Apr 9, 2001·Nitric Oxide : Biology and Chemistry·J C PreiserJ L Vincent
Oct 3, 2013·The Veterinary Journal·Mar Costa-Hurtado, Virginia Aragon
Sep 13, 2006·Veterinary Pathology·A L Pereira-SuárezR Mancilla
Sep 18, 1999·Veterinary Pathology·J M FliggerT W Jungi
Apr 29, 2014·Psychological Reports·Seydi Ahmet SaticiAhmet Akin
Apr 17, 1998·Immunopharmacology and Immunotoxicology·M A PanaroO Brandonisio
Jan 10, 2018·Frontiers in Immunology·Jamal Hussen, Hans-Joachim Schuberth

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