Differential regulation of integrin-mediated proplatelet formation and megakaryocyte spreading

Journal of Cellular Physiology
R M Leven

Abstract

Guinea pig bone marrow megakaryocytes were cultured on a type I rat tail collagen gel which stimulated proplatelet formation. Proplatelet formation was inhibited by monoclonal antibody LM609 to the alpha v beta 3 integrin (VnR), but not by monoclonal antibodies to the alpha 5, alpha 6, beta 1, or IIb beta 3(GPIIb-IIIa) integrin proteins. Megakaryocytes cultured on a plastic surface and stimulated with thrombin undergo a spreading and an adhesion reaction. This reaction is blocked in a dose-dependent manner by the tetrapeptide RGDS and by the monoclonal antibody PG2 to the GPIIb-IIIa integrin, but not by the monoclonal antibody LM609 to the VnR. Immunoprecipitation and affinity chromatography experiments demonstrate that guinea pig megakaryocytes have distinct GPIIb-IIIa and VnR integrins with similar electrophoretic mobility. Spreading was significantly inhibited in a dose-dependent fashion by drugs which elevate cellular cyclic AMP, including forskolin, dibutyryl cAMP, and isobutylmethylxanthine. In contrast to spreading, megakaryocyte proplatelet formation was stimulated by these agents in a dose-dependent manner. Megakaryocyte spreading was stimulated by the protein kinase C (PKC) activator phorbol myristate acetate (PMA) an...Continue Reading

References

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Citations

Jan 17, 2002·International Journal of Hematology·J Li, D J Kuter
Jul 19, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R A PeregoE Morra
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Sep 29, 2007·Critical Reviews in Oncology/hematology·Lin WangMing Hou
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Dec 18, 2007·Biochemical and Biophysical Research Communications·Satoshi TamaruMasakatsu Nishikawa
Oct 12, 2017·Journal of Natural Medicines·Takahiro SatoIkuo Morita
Jul 9, 2003·The Journal of Biological Chemistry·Hiroshi KojimaAkira Shibuya
Apr 3, 2001·The Journal of Laboratory and Clinical Medicine·M SchattnerM A Lazzari

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