Differential regulation of key stages in early corneal wound healing by TGF-beta isoforms and their inhibitors

Investigative Ophthalmology & Visual Science
Louise M CarringtonMike Boulton

Abstract

Inhibition of TGF-beta reduces myofibroblast differentiation and fibrosis in the cornea. Determining the actions of distinct TGF-beta isoforms and their inhibitors during early corneal wound healing is an essential step in guiding therapeutic intervention. Bovine serum-free corneal cell and wounded organ cultures were challenged with a range of concentrations of TGF-beta1, -beta2, and -beta3; IL-10; and neutralizing human monoclonal antibodies (mAbs) against TGF-beta1 (CAT-192) or -beta2, (CAT-152). Cultures were assessed for re-epithelialization, proliferation (cell counts and cresyl violet assay), morphology (histologic examination), repopulation of the area under the wound, and myofibroblast transformation (alpha-smooth muscle actin) between 0 and 5 days. TGF-beta1 delayed re-epithelialization, increased repopulation of the stroma, increased keratocyte proliferation and was the only isoform to promote myofibroblast differentiation. The anti-TGF-beta1 mAb, CAT-192 promoted re-epithelialization and reduced repopulation of the stroma. Exogenous TGF-beta3 had little effect on re-epithelialization but reduced repopulation of the stroma. IL-10 promoted corneal re-epithelialization at low doses but inhibited this response at high d...Continue Reading

Citations

Aug 21, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Henning ThomasenDaniel Meller
Jun 26, 2010·Japanese Journal of Ophthalmology·Winston W-Y Kao, Chia-Yang Liu
Oct 12, 2010·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Marion K GordonPatrick J Sinko
Feb 15, 2014·Advances in Wound Care·Kenneth W FinnsonAnie Philip
May 18, 2007·Molecular Biology of the Cell·Audrey M BernsteinSandra K Masur
Aug 21, 2010·Eye & Contact Lens·Winston W-Y Kao
Dec 25, 2010·Journal of Cataract and Refractive Surgery·Shengli MiChristina S Kamma-Lorger
Mar 20, 2016·Experimental Eye Research·Xiaoqing GuoJames D Zieske
May 24, 2011·Journal of Tissue Engineering and Regenerative Medicine·D KaramichosJ D Zieske
Nov 21, 2012·Ocular Immunology and Inflammation·Hassan GhasemiSoghrat Faghihzadeh
Jun 28, 2014·Experimental Eye Research·Sriniwas SriramGregory Schultz
May 25, 2007·Burns : Journal of the International Society for Burn Injuries·Abdo JurjusRania A Tohme
Jun 2, 2009·Brain Research Bulletin·Yureeda QaziBalamurali K Ambati
Jan 1, 2012·Journal of Functional Biomaterials·Samantha L WilsonYing Yang
Jul 28, 2013·Journal of Functional Biomaterials·Dimitrios KaramichosJames D Zieske
Aug 22, 2018·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Mai Abd El-MeguidMostafa K El Awady
Dec 8, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Aihua MaJulie Albon
Aug 24, 2018·Scientific Reports·Pouriska B KivananyW Matthew Petroll
Nov 19, 2013·Molecular and Cellular Biochemistry·Xu SunJun Li
Sep 1, 2008·Annals of Dermatology·Moon Chul JungNack In Kim
Dec 22, 2016·Frontiers in Physiology·Pui WongGary Tse
Jan 21, 2021·Toxicologic Pathology·Meg Ferrell RamosSharmila Masli
Sep 4, 2020·Experimental Eye Research·Winston W-Y Kao
Oct 13, 2017·Experimental Eye Research·Hidetaka MiyagiChristopher J Murphy
May 16, 2017·International Immunopharmacology·Jong Won KimBumseok Kim
Mar 17, 2011·The American Journal of Pathology·Geniece M LehmannRichard P Phipps
Jun 11, 2017·Experimental Eye Research·Sriniwas SriramJames D Zieske
May 6, 2021·Journal of Cellular and Molecular Medicine·Núria Nieto-NicolauRicardo P Casaroli-Marano
Sep 2, 2021·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Selikem NuwormegbeSun Woong Kim
Jan 10, 2009·Experimental Eye Research·Christina S Kamma-LorgerKeith M Meek
Sep 8, 2009·Brain Research Bulletin·Fu-Shin X YuJenny Huang
May 24, 2011·Current Biology : CB·Toru MaedaTakao Sakai

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