Differential regulation of Listeria monocytogenes internalin and internalin-like genes by sigmaB and PrfA as revealed by subgenomic microarray analyses.

Foodborne Pathogens and Disease
Patrick McGannK J Boor

Abstract

The Listeria monocytogenes genome contains more than 20 genes that encode cell surface-associated internalins. To determine the contributions of the alternative sigma factor sigma(B) and the virulence gene regulator PrfA to internalin gene expression, a subgenomic microarray was designed to contain two probes for each of 24 internalin-like genes identified in the L. monocytogenes 10403S genome. Competitive microarray hybridization was performed on RNA extracted from (i) the 10403S parent strain and an isogenic Delta sigB strain; (ii) 10403S and an isogenic Delta prfA strain; (iii) a (G155S) 10403S derivative that expresses the constitutively active PrfA (PrfA*) and the Delta prfA strain; and (iv) 10403S and an isogenic Delta sigB Delta prfA strain. Sigma(B)- and PrfA-dependent transcription of selected genes was further confirmed by quantitative reverse-transcriptase polymerase chain reaction. For the 24 internalin-like genes examined, (i) both sigma(B) and PrfA contributed to transcription of inlA and inlB, (ii) only sigma(B) contributed to transcription of inlC2, inlD, lmo0331, and lmo0610; (iii) only PrfA contributed to transcription of inlC and lmo2445; and (iv) neither sigma(B) nor PrfA contributed to transcription of the ...Continue Reading

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J C PiffarettiJ Rocourt
Apr 1, 1993·Molecular Microbiology·A CamilliD A Portnoy
Jan 1, 1997·The Veterinary Journal·J C Low, W Donachie
Dec 1, 1996·Nature Biotechnology·D J LockhartE L Brown
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·W W Navarre, O Schneewind
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·M MarinoP Ghosh
Dec 2, 2000·Nature Biotechnology·D W SelingerG M Church
Jul 4, 2001·Clinical Microbiology Reviews·J A Vázquez-BolandJ Kreft
Oct 27, 2001·Science·P GlaserP Cossart
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Apr 26, 2002·Trends in Microbiology·Didier CabanesPascale Cossart
May 30, 2002·Nucleic Acids Research·Angela RelógioJuan Valcárcel
Jun 18, 2002·Infection and Immunity·Celine A NadonKathryn J Boor
Sep 18, 2003·Journal of Bacteriology·Mark J KazmierczakMartin Wiedmann
Sep 23, 2003·Nucleic Acids Research·Paul K TanMargaret C Cam
Nov 5, 2003·Methods : a Companion to Methods in Enzymology·Gordon K Smyth, Terry Speed
Dec 23, 2003·Infection and Immunity·Anna I BakardjievDaniel A Portnoy
Apr 7, 2004·Molecular Microbiology·Svetlana ErmolaevaJosé A Vázquez-Boland
Apr 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Marc LecuitPascale Cossart

❮ Previous
Next ❯

Citations

Mar 19, 2014·International Journal of Food Microbiology·V B RibeiroM T Destro
Feb 24, 2010·Infection and Immunity·Nicolas PersonnicHélène Bierne
Mar 4, 2009·Infection and Immunity·Juliane OllingerTeresa M Bergholz
Sep 29, 2011·International Journal of Molecular Sciences·Benjamin IzarTorsten Hain
Dec 3, 2014·Journal of Immunological Methods·Robert W GeneMehdi Arbabi-Ghahroudi
Feb 26, 2011·Immunological Reviews·Fabrizia StavruPascale Cossart
Sep 15, 2012·Journal of Medical Microbiology·Angela PayneJanet R Donaldson
Sep 10, 2017·Applied and Environmental Microbiology·Dafni Katerina PaspaliariMarianne Halberg Larsen
Sep 6, 2019·Microbiology and Molecular Biology Reviews : MMBR·Ahmed GaballaKathryn J Boor
Nov 9, 2018·Animal Frontiers : the Review Magazine of Animal Agriculture·Peter W Cook, Kendra K Nightingale

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.