Differential regulation of methionine adenosyltransferase in superantigen and mitogen stimulated human T lymphocytes.

The Journal of Biological Chemistry
H L LeGrosM Kotb

Abstract

Superantigens interact with the T cell receptor for antigen (TCR) and are, therefore, more physiological stimulators of T lymphocytes than nonspecific polyclonal T cell mitogens. The effects of these two classes of T cell stimulators on methionine adenosyltransferase (MAT) and S-adenosylmethionine (AdoMet) levels were investigated. Activation of resting human peripheral blood T lymphocytes by the mitogen phytohemagglutinin (PHA) or the superantigen staphylococcal enterotoxin B (SEB) caused a 3- to 6-fold increase in MAT II specific activity. Although the proliferative response was higher in cultures stimulated with PHA compared with SEB, MAT II activity was comparable in both cultures. Both stimuli caused down-regulation of the MAT 68-kDa lambda subunit expression and induced a comparable increase in the expression of the catalytic alpha2/alpha2' subunit mRNA and protein. However, in superantigen-stimulated cells, the expression of the noncatalytic beta subunit was down-regulated and virtually disappeared by 72 h post-stimulation; whereas, no change in the expression of this subunit was noted in PHA-stimulated cells. Thus, at 72 h following stimulation, PHA-stimulated cells expressed MAT II alpha2/alpha2' and beta subunits whil...Continue Reading

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Citations

Oct 28, 2008·Cellular and Molecular Life Sciences : CMLS·G D Markham, M A Pajares
Oct 31, 1998·Biochimica Et Biophysica Acta·B Langkamp-HenkenM Kotb
May 25, 2011·Protein Expression and Purification·Francisco GarridoMaría A Pajares
Jan 1, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Komal RamaniShelly C Lu
May 30, 2017·Nature Chemical Biology·Casey L QuinlanStephan K Grant
Dec 1, 2017·Antioxidants & Redox Signaling·María A Pajares, Dolores Pérez-Sala
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jose M MatoMatias A Avila
Apr 12, 2020·Biomolecules·Peter Kaiser
Feb 5, 2021·Cancers·Ali Mehdi, Shafaat A Rabbani
May 18, 2020·Immunology·Chan-Wang Jerry Lio, Stanley Ching-Cheng Huang
Sep 1, 1997·Archives of Biochemistry and Biophysics·A M GellerM Y Kotb
Jul 31, 2001·Biochimica Et Biophysica Acta·A B HalimM Kotb

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