PMID: 9440520Jan 24, 1998Paper

Differential regulation of multiple flagellins in Vibrio cholerae

Journal of Bacteriology
K E Klose, J J Mekalanos

Abstract

Vibrio cholerae, the causative agent of the human diarrheal disease cholera, is a motile bacterium with a single polar flagellum. Motility has been implicated as a virulence determinant in some animal models of cholera, but the relationship between motility and virulence has not yet been clearly defined. We have begun to define the regulatory circuitry controlling motility. We have identified five V. cholerae flagellin genes, arranged in two chromosomal loci, flaAC and flaEDB; all five genes have their own promoters. The predicted gene products have a high degree of homology to each other. A strain containing a single mutation in flaA is nonmotile and lacks a flagellum, while strains containing multiple mutations in the other four flagellin genes, including a flaCEDB strain, remain motile. Measurement of fla promoter-lacZ fusions reveals that all five flagellin promoters are transcribed at high levels in both wild-type and flaA strains. Measurement of the flagellin promoter-lacZ fusions in Salmonella typhimurium indicates that the promoter for flaA is transcribed by the sigma54 holoenzyme form of RNA polymerase while the flaE, flaD, and flaB promoters are transcribed by the sigma28 holoenzyme. These results reveal that the V. c...Continue Reading

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Citations

Jun 13, 2000·Journal of Bacteriology·Y K Kim, L L McCarter
Jul 21, 2004·Journal of Bacteriology·Crystal M LaurianoKarl E Klose
Feb 20, 2007·The Journal of Biological Chemistry·Jason T PrattAndrew Camilli
Sep 22, 2009·Journal of Bacteriology·Manuel MoisiStefan Schild
Oct 2, 2019·Nature Microbiology·Gustavo Espinoza-VergaraDiane McDougald
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·L L McCarter
Feb 21, 2002·World Journal of Gastroenterology : WJG·W S JiD M Fan
Jan 5, 2008·Infection and Immunity·Sang Sun Yoon, John J Mekalanos
Dec 9, 2004·DNA and Cell Biology·Shah M FaruqueJohn J Mekalanos
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Haopeng RuiMatthew K Waldor
Jul 9, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sanjay Dey, Jhimli Dasgupta
Apr 5, 2020·Biomolecules·Seiji KojimaMichio Homma
Feb 23, 1999·Molecular Microbiology·C A Murphy, R Belas
Nov 3, 2005·Molecular Microbiology·Michael G ProutyKarl E Klose
Aug 23, 2005·Infection and Immunity·Anna D Tischler, Andrew Camilli
Apr 20, 2005·Journal of Bacteriology·Nidia E Correa, Karl E Klose
Sep 26, 2006·Molecular & Cellular Proteomics : MCP·Juan Xicohtencatl-CortésJorge A Girón
Aug 3, 2013·MicrobiologyOpen·Caitlin A BrennanEdward G Ruby
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·C C Häse, J J Mekalanos

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