PMID: 9164840May 15, 1997Paper

Differential regulation of natriuretic peptide receptors on ciliary body epithelial cells

The Biochemical Journal
R B Crook, A T Chang

Abstract

Atrionatriuretic peptide (ANP) lowers intraocular pressure in the eyes of humans and rabbits. We examined the effects of natriuretic peptides on cGMP formation and 125I-labelled-ANP binding to cultured cells derived from ciliary body epithelium, the site of aqueous humour formation in the eye. ANP, brain natriuretic peptide (BNP) and C-natriuretic peptide (CNP) at 1 microM stimulated cGMP formation 8.2(+/-1.2)-fold, 4.8(+/-0.6)-fold and 87.3(+/-12.1)-fold respectively. 125I-ANP bound to intact cells at a single site, with a dissociation constant KD=0.30+/-0.01 nM. BNP was as effective as ANP in displacing 125I-ANP, whereas CNP displaced label with a slightly higher IC50. 125I-ANP binding was displaced >95% by c-ANP, a specific ligand for natriuretic peptide C receptors (NPR-C). Cross-linking of 125I-ANP to cells labelled predominantly a protein of Mr 62000. These data suggest that 125I-ANP binding was primarily to NPR-C, whereas cGMP stimulation occurred primarily via natriuretic peptide B receptors (NPR-B). Vasopressin and histamine, both activators of the inositol phosphate/diacylglycerol phosphate pathway in non-pigmented ciliary epithelial cells, inhibited CNP stimulation of guanylate cyclase (NPR-B) and 125I-ANP binding (N...Continue Reading

Citations

May 14, 2004·American Journal of Physiology. Cell Physiology·Pawel FidzinskiMiguel Coca-Prados
Sep 24, 2004·The Journal of Membrane Biology·C W Do, M M Civan
Mar 6, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Matthias SchmittMichael P Frenneaux
Dec 19, 2002·Endocrinology·Sarah E Abbey, Lincoln R Potter
Jun 21, 2006·Molecular and Cellular Biochemistry·William R GowerSuzanne M Solivan
May 1, 1999·Biochemical and Biophysical Research Communications·J Ortego, M Coca-Prados
Feb 5, 1999·European Journal of Pharmacology·R Fernández-DurangoR Bernal
Apr 7, 1999·Progress in Retinal and Eye Research·M Coca-PradosJ Ortego
May 5, 2018·International Journal of Cardiology·Katarzyna KodziszewskaPrzemyslaw Leszek

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