Differential regulation of platelet aggregation and aminophospholipid exposure by calpain

British Journal of Haematology
Christilla Bachelot-LozaF Rendu

Abstract

Aggregation, exposure of procoagulant phospholipids and shedding of microparticles are platelet responses that depend on activating conditions. To determine how these different responses are interconnected, we simultaneously measured fibrinogen (Fg) binding and aminophospholipid exposure on activated platelets by means of flow cytometry. Low calcium ionophore (A23187) concentrations induced Fg binding but not annexin V binding. In contrast, high A23187 concentrations induced annexin V binding but not Fg binding. Collagen, both alone and in the presence of thrombin, induced both Fg and annexin V binding. Dual labelling was found on 38 +/- 9% of platelets stimulated by thrombin plus collagen. The regulatory role of calpain in these platelet functions was investigated. When calpain was partially inhibited by 2 microg/ml calpeptin, Fg binding still occurred but aminophospholipid exposure was limited. By contrast, complete inhibition of calpain by 100 microg/ml calpeptin or E64d decreased Fg binding but enhanced aminophospholipid exposure. In these latter conditions, cytosolic calcium-extruding systems were inhibited. The results suggest that (i) conditions that favour aminophospholipid exposure tend to decrease the aggregation proc...Continue Reading

References

Nov 17, 1995·The Journal of Biological Chemistry·S SheriffG A Grabowski
Apr 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·H AriyoshiE W Salzman
Apr 1, 1996·Archives of Biochemistry and Biophysics·M InomataS Kawashima
Aug 1, 1996·European Journal of Biochemistry·J M PasquetA T Nurden
Jun 13, 1997·The Journal of Biological Chemistry·W L DeanT C Vanaman
Oct 6, 1997·The Journal of Biological Chemistry·S M SchoenwaelderS P Jackson
Jan 31, 1998·The Journal of Biological Chemistry·A HuttenlocherA F Horwitz
Nov 14, 1998·Biochemical and Biophysical Research Communications·H FaletF Rendu
Sep 25, 1999·The Journal of Biological Chemistry·D A CalderwoodM H Ginsberg
Dec 14, 1999·The Journal of Biological Chemistry·K CroceD A Potter
Aug 12, 2000·Platelets·S M SchoenwaelderS P Jackson
Mar 10, 2001·Molecular and Cellular Biology·M AzamA H Chishti
May 31, 2001·The Journal of Biological Chemistry·B YanM H Ginsberg
Apr 5, 2002·The Journal of Biological Chemistry·David A CalderwoodMark H Ginsberg
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·J-M Freyssinet
Oct 4, 2003·Science·Seiji TadokoroDavid A Calderwood
Feb 26, 2004·The Journal of Biological Chemistry·Diane E RobertsRatna Bose
May 8, 2004·The Journal of Biological Chemistry·Suhasini Kulkarni, Shaun P Jackson
Jun 19, 2004·Blood·Sanford J Shattil, Peter J Newman
Feb 1, 2005·Cell·Daniele BanoPierluigi Nicotera

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Citations

Jan 1, 2009·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Malvina CrespinMichaëla Fontenay
Jun 3, 2008·Journal of Thrombosis and Haemostasis : JTH·J M E M CosemansJ W M Heemskerk
Oct 31, 2012·Journal of Thrombosis and Haemostasis : JTH·J W M HeemskerkJ M E M Cosemans
Jan 19, 2011·British Journal of Haematology·Yukinori KozumaHaruhiko Ninomiya
Jun 25, 2016·Biointerphases·Alessia DonatiIlya Reviakine

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