Differential regulation of proinflammatory mediators following LPS- and ATP-induced activation of monocytes from patients with antiphospholipid syndrome

BioMed Research International
Anush MartirosyanGayane Manukyan

Abstract

Antiphospholipid syndrome (APS) is an acquired autoimmune disorder characterized by recurrent thrombosis and pregnancy morbidity in association with the presence of antiphospholipid antibodies. Growing evidence supports the involvement of monocytes in APS pathogenesis. Inflammatory activation of monocytes promotes thrombus formation and other APS complications. However, mechanisms underlying their activation are poorly investigated. We aimed to determine transcriptional activity of monocytes after exposing them to low concentrations of lipopolysaccharide (LPS) and LPS + adenosine triphosphate (ATP) using comparative qRT-PCR. The results showed that LPS significantly increased transcriptional levels of TLR2, IL-23, CCL2, CXCL10, IL-1β, and IL-6 in APS cells, while, in cells from healthy donors, LPS resulted in IL-6 and STAT3 elevated mRNAs. Double stimulation of the cells resulted in decreased mRNA levels of NLRP3 in monocytes isolated from healthy donors and CCL2, IL-1β in APS cells. By contrast, TLR2 mRNAs were elevated in both investigated groups after culture of the cells with LPS + ATP. Thus, the findings indicate increased sensitivity of APS cells to LPS that may contribute to thrombus formation and enhance development or ...Continue Reading

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Citations

Sep 1, 2015·Autoimmunity Reviews·Lucas L van den HoogenRonald H W M Derksen
Jul 30, 2019·Frontiers in Immunology·Anush MartirosyanGayane Manukyan
Jul 7, 2020·Journal of Thrombosis and Thrombolysis·Bruna de Moraes MazettoFernanda Andrade Orsi
Feb 5, 2021·Biomedicines·Ilaria MormileFrancesca Wanda Rossi

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Methods Mentioned

BETA
blood collection
density gradient centrifugation
flow cytometry
Enzyme-linked immunosorbent assay
ELISA

Software Mentioned

Graph Pad
Rotor
Gene
Graph Pad Prism

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