Differential regulation of protein-tyrosine phosphatases by integrin alpha IIb beta 3 through cytoskeletal reorganization and tyrosine phosphorylation in human platelets.

The Journal of Biological Chemistry
Y EzumiM Okuma

Abstract

The major platelet integrin alpha IIb beta 3 (glycoprotein IIb-IIIa) has been implicated in the regulation of tyrosine phosphorylation and dephosphorylation in activated platelets. To investigate the mechanisms of the alpha IIb beta 3-dependent tyrosine dephosphorylation, normal platelets or thrombasthenic platelets lacking alpha IIb beta 3 were stimulated with thrombin and fractionated into Triton X-100-soluble or -insoluble subcellular matrices. We then examined the kinetics of the tyrosine-phosphorylated proteins and distribution of protein-tyrosine phosphatases in these fractions and whole cell lysates. First, alpha IIb beta 3-dependent tyrosine dephosphorylation was recovered mainly in the cytoskeleton with similar kinetics to the whole cell lysate. Second, protein-tyrosine phosphatase (PTP) 1B and its cleaved 42-kDa form were associated with the cytoskeleton in an aggregation-dependent manner, whereas association of PTP1C with the cytoskeleton was regulated differentially both by thrombin stimulation and by alpha IIb beta 3-mediated aggregation. Several calpain inhibitors did not affect either tyrosine phosphorylation and dephosphorylation or relocation of PTP1B, but they did inhibit cleavage of PTP1B. Cytochalasin D bloc...Continue Reading

References

Dec 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·R M FreemanB G Neel
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M M HuangJ S Brugge
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J PlutzkyR D Rosenberg
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D E CoolE G Krebs
Jan 1, 1990·Annual Review of Cell Biology·N Kieffer, D R Phillips
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A GoldenJ S Brugge
Sep 1, 1988·Molecular and Cellular Biology·J E Ferrell, G S Martin
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Golden, J S Brugge
Jun 30, 1988·Biochemical and Biophysical Research Communications·T TsujinakaT Mori
Dec 30, 1988·Biochemical and Biophysical Research Communications·S MehdiP Bey
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Ferrell, G S Martin
Aug 1, 1986·Journal of Pharmacobio-dynamics·M TamaiK Hanada
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S AhmadS H Shen
Jul 15, 1993·Biochemical and Biophysical Research Communications·H TakayamaM Okuma
Feb 1, 1993·The Journal of Cell Biology·R L Juliano, S Haskill

❮ Previous
Next ❯

Citations

Feb 13, 2001·Journal of Neuroscience Research·P PathreJ Balsamo
May 2, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jack LilienGang Xu
Jun 20, 2007·Molecular and Cellular Biology·Shafi M KuchayAthar H Chishti
Jan 10, 1997·The Journal of Biological Chemistry·G HuyerC Ramachandran
Jun 19, 2004·The Journal of Biological Chemistry·K Vinod VijayanPaul F Bray
Apr 21, 2005·Protein Science : a Publication of the Protein Society·Chad J BlameyBrian J Bahnson
Jul 13, 2002·Journal of Cellular Physiology·Hao ChengAngel Alonso
Sep 15, 2001·FEBS Letters·S MukhopadhyayD Dash
Dec 1, 2006·The Journal of Biological Chemistry·Kristen M PichaLawrence P Wennogle
Mar 31, 2012·Journal of Thrombosis and Haemostasis : JTH·S M KuchayA H Chishti
May 28, 1998·The Journal of Biological Chemistry·F M Donovan, D D Cunningham
May 31, 2001·Clinical and Experimental Pharmacology & Physiology·S M DopheideS P Jackson
Apr 9, 2004·The Journal of Biological Chemistry·Siang-Yo LinBeatrice Haimovich
Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tracy L DeemJoan M Cook-Mills
Oct 13, 2010·Archives of Biochemistry and Biophysics·Mariana A CalleroAlcira B Nesse

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.