PMID: 12493702Dec 21, 2002Paper

Differential regulation of steroid hormone biosynthesis in R2C and MA-10 Leydig tumor cells: role of SR-B1-mediated selective cholesteryl ester transport

Biology of Reproduction
Rekha M RaoD M Stocco

Abstract

The rat R2C Leydig tumor cell line is constitutively steroidogenic in nature, while the mouse MA-10 Leydig tumor cell line synthesizes large amounts of steroids only in response to hormonal stimulation. Earlier studies showed abundant cAMP-independent steroid production and constitutive expression of steroidogenic acute regulatory (StAR) protein in R2C cells. The objective of the current study was to identify possible genetic alterations in the R2C cell line responsible for rendering it a constitutively steroidogenic cell line, especially those that might have altered its cholesterol homeostatic mechanisms. Measurement of the levels of cholesterol esters and free cholesterol, precursors for steroidogenesis, indicated that R2C mitochondria were fourfold enriched in free cholesterol content compared with MA-10 mitochondria. In addition to the previously demonstrated increased expression of StAR protein, we show that R2C cells possess marginally enhanced protein kinase A activity, exhibit higher capacity to take up extracellular cholesterol esters, and express much higher levels of scavenger receptor-type B class 1 (SR-B1) and hormone sensitive lipase (HSL). These observations suggest that the high level of steroid biosynthesis in...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Ascoli, D Puett
Nov 12, 1990·Biochimica Et Biophysica Acta·Salman AzharE Reaven
Apr 4, 1986·Science·M S Brown, J L Goldstein
Oct 1, 1995·Molecular Endocrinology·B J ClarkD M Stocco
Jul 5, 1996·The Journal of Biological Chemistry·E ReavenSalman Azhar
Jun 1, 1996·Endocrine Reviews·D M Stocco, B J Clark
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·F ArakaneJ F Strauss
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·H S BoseW L Miller
Sep 11, 1999·Current Opinion in Lipidology·D L WilliamsG H Rothblat
Jan 12, 2000·Molecular and Cellular Endocrinology·X Wang, D M Stocco
Dec 9, 2000·Biochimica Et Biophysica Acta·L K Christenson, J F Strauss
Feb 22, 2001·Annual Review of Physiology·D M Stocco
Jun 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·Pulak R MannaI Huhtaniemi
Jul 21, 2001·Molecular Endocrinology·D M Stocco
Sep 19, 2001·Current Opinion in Lipidology·D L Silver, A R Tall

Citations

Aug 28, 2012·Advanced Drug Delivery Reviews·Marina G DamianoC Shad Thaxton
Jan 11, 2012·Molecular Pharmacology·Masami Shimizu-AlbergineJoseph A Beavo
Oct 1, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·María V T LoboAntonia Martín-Hidalgo
Jul 18, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Rajani M GeorgeJeanne Wilson-Rawls
Apr 13, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Pergentino BalbuenaRebecca A Clewell
Jul 17, 2016·The Journal of Endocrinology·Vimal Selvaraj, Lan N Tu
Nov 16, 2004·Molecular and Cellular Endocrinology·Nafis A Rahman, I Huhtaniemi
Oct 13, 2016·Frontiers in Pharmacology·Maneesha A Rajora, Gang Zheng
Feb 29, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·David GunnarssonGunnar Selstam
Sep 17, 2014·Molecular and Cellular Biology·Houssein S AbdouJacques J Tremblay
Mar 22, 2007·American Journal of Physiology. Endocrinology and Metabolism·Guey-Shyang HwangPaulus S Wang
Jun 23, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kristina PogrmicRadmila Kovacevic
Jul 22, 2014·Clinical and Translational Science·Kai Yin, Devendra K Agrawal
Aug 15, 2015·Endocrine·Pulak R MannaKevin Pruitt
Apr 24, 2010·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S DebS M Bandiera
Jul 30, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kristina Pogrmic-MajkicRadmila Kovacevic
Nov 7, 2016·The Journal of Biological Chemistry·Sathvika VenugopalVassilios Papadopoulos
Dec 12, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yijun MeiMin Ren
Oct 17, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Luona WenWeibin Bai

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