Differential regulation of telomerase in endothelial cells by fibroblast growth factor-2 and vascular endothelial growth factor-a: association with replicative life span

Annals of the New York Academy of Sciences
Elisabeth TrivierJorge D Erusalimsky

Abstract

In cultured human umbilical vein endothelial cells (HUVECs), fibroblast growth factor-2 (FGF-2), but not vascular endothelial growth factor-A (VEGF-A), upregulates telomerase activity. Here, we examined the functional significance of this differential regulation on the replicative life span of HUVECs. HUVECs were serially passaged until senescence under four different conditions: (1) EGM-2, a medium containing both VEGF-A and FGF-2; (2) basal medium (BM), consisting of EGM-2 devoid of FGF-2 and VEGF-A; (3) BM supplemented with FGF-2; and (4) BM supplemented with VEGF-A. Cells cultured in BM demonstrated decreased growth rate and ceased to proliferate at approximately 15 population doublings (PDs), whereas those cultured with VEGF-A alone initially proliferated vigorously but arrested growth abruptly at a PD level comparable with cultures grown in BM. In contrast, cells maintained in EGM-2 or in BM/FGF-2 attained a normal replicative life span (approximately 40 PDs). These differences in replicative behavior were reflected by the early appearance of a senescent phenotype in cultures grown in BM or BM/VEGF-A. HUVECs grown in the presence of VEGF-A alone have a decreased life span compared with cultures maintained with FGF-2. This...Continue Reading

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Citations

Feb 8, 2008·Molecular Biology of the Cell·Anneke E SchwobKarina F Meiri
Jul 17, 2008·Seminars in Dialysis·Marie-Luise Gross, Eberhard Ritz
Nov 28, 2008·Journal of Applied Physiology·Jorge D Erusalimsky
Apr 7, 2010·Ageing Research Reviews·Jun-Ping LiuHe Li
Jul 22, 2009·Ageing Research Reviews·María Dolores HerreraMaria Alvarez de Sotomayor
Jan 1, 2008·Experimental Cell Research·Jen-Chine WuWoei-Yau Kao
Jun 15, 2007·Gynecologic Oncology·Yira BermudezPatricia A Kruk
Oct 22, 2008·Experimental Physiology·Jorge D Erusalimsky, Chris Skene
Jun 24, 2011·PloS One·Magomed KhaidakovJawahar L Mehta
Mar 23, 2002·Experimental Gerontology·Annette M MüllerKlaus-Michael Müller
Dec 21, 2010·Methods : a Companion to Methods in Enzymology·Jin-Na Min, Cam Patterson
Dec 22, 2017·International Journal of Molecular Sciences·Thomas AschacherMichael Bergmann
Nov 24, 2006·Circulation Research·José J Fuster, Vicente Andrés
Feb 3, 2005·The Journal of Biological Chemistry·Germana ZaccagniniAntonella Farsetti

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