Differential regulation of the nuclear factor-κB pathway by rabbit antithymocyte globulins in kidney transplantation

Transplantation
Mariana UrbanovaOndrej Viklicky

Abstract

Induction therapy is associated with excellent short-term kidney graft outcome. The aim of this study was to evaluate differences in the intragraft transcriptome after successful induction therapy using two rabbit antithymocyte globulins. The expression of 376 target genes involved in tolerance, inflammation, T- and B-cell immune response, and apoptosis was evaluated using the quantitative real-time reverse-transcriptase polymerase chain reaction (2(-ΔΔCt)) method in kidney graft biopsies with normal histological findings and stable renal function, 3 months posttransplantation after induction therapy with Thymoglobulin, ATG-Fresenius S (ATG-F), and a control group without induction therapy. The transcriptional pattern induced by Thymoglobulin differed from ATG-F in 18 differentially expressed genes. Down-regulation of genes involved in the nuclear factor-κB pathway (TLR4, MYD88, and CD209), costimulation (CD80 and CTLA4), apoptosis (NLRP1), chemoattraction (CCR10), and dendritic cell function (CLEC4C) was observed in the biopsies from patients treated with Thymoglobulin. A hierarchical clustering analysis clearly separated the Thymoglobulin group from the ATG-F group, while the control group had a similar profile as the Thymogl...Continue Reading

References

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Citations

Jul 24, 2013·Expert Opinion on Biological Therapy·Afshin GharekhaniHossein Khalili
May 6, 2016·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Alena ParikovaRaymond T Krediet
Jun 7, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ondrej ViklickyIrena Brabcova
Sep 8, 2016·American Journal of Physiology. Endocrinology and Metabolism·Irena TýcováOndřej Viklický

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