Differential regulation of the two neuronal nitric-oxide synthase gene promoters by the Oct-2 transcription factor.

The Journal of Biological Chemistry
Z DeansD S Latchman

Abstract

The Oct-2 transcription factor has been shown previously to repress both the cellular tyrosine hydroxylase and the herpes simplex virus immediate-early genes in neuronal cells. Here we identify the gene encoding the neuronal nitric-oxide synthase (nNOS) as the first example of a gene activated in neuronal cells by Oct-2. The levels of the nNOS mRNA and protein are greatly reduced in neuronal cell lines in which Oct-2 levels have been reduced by an antisense method, although these cells have enhanced levels of tyrosine hydroxylase. Moreover, the nNOS gene regulatory region is activated by Oct-2 expression vectors upon cotransfection into both neuronal and non-neuronal cells, and this response is dependent upon a 20-amino acid region within the COOH-terminal activation domain of Oct-2. Of the two closely linked promoters that drive nNOS gene expression, only the downstream 5.1 promoter is activated by Oct-2, whereas the 5.2 promoter is unaffected. These effects are discussed in terms of the potential role of Oct-2 in regulating nNOS expression in the nervous system.

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Citations

Apr 13, 1999·Journal of Cellular Physiology·D S Latchman
Jan 14, 1998·Brain Research. Molecular Brain Research·Z C DeansD S Latchman
Nov 9, 2005·The Journal of Clinical Investigation·Michael E WardPhilip A Marsden
Dec 27, 2005·Progress in Neurobiology·Julie Y H ChanSamuel H H Chan
Apr 1, 1999·Nitric Oxide : Biology and Chemistry·J P BoisselU Förstermann
Oct 6, 2000·Nitric Oxide : Biology and Chemistry·E M GattoJ J Poderoso
Sep 4, 2009·American Journal of Physiology. Cell Physiology·Florian NaglDieter Saur
Jun 10, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M J SolhaugK W Dong
Jan 24, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dieter SaurHans-Dieter Allescher
Sep 18, 1997·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·B C Kone

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