Differential regulation of TRPV4 mRNA levels by acclimation salinity and extracellular osmolality in euryhaline tilapia

General and Comparative Endocrinology
A P SealeE Gordon Grau

Abstract

Prolactin (PRL) cells of the euryhaline Mozambique tilapia, Oreochromis mossambicus, are osmoreceptors. Hyposmotically-induced PRL release is mediated by the inward movement of extracellular Ca(2+) through a stretch-activated Ca(2+) channel, which has been recently identified as the transient receptor potential vanilloid 4 (TRPV4). In the present study, changes in plasma PRL, as well as PRL and TRPV4 mRNA expression from the rostral pars distalis (RPD), were measured in fish transferred from seawater (SW) to fresh water (FW) and in fish transferred from FW to SW. The in vitro effects of osmolality on PRL release and on PRL and TRPV4 mRNA expression in dispersed PRL cells were compared between fish adapted to SW and FW. Both the release and expression of PRL fell when fish were transferred to SW and rose when fish were transferred to FW. By contrast, TRPV4 expression increased by 48h after fish were transferred from FW to SW and declined as early as 6h after transfer from SW to FW. A similar pattern was observed in vitro where TRPV4 expression responded positively to an increase in medium osmolality while PRL expression declined. Incubation with the Ca(2+) ionophore, A23187, and the phosphodiesterase inhibitor, IBMX, stimulated ...Continue Reading

References

Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J L SpeckerH A Bern
May 7, 1999·Environmental Health Perspectives·G E Kass, S Orrenius
Mar 9, 2002·General and Comparative Endocrinology·Lori A Manzon
Oct 24, 2002·General and Comparative Endocrinology·Andre P SealeE Gordon Grau
Jan 24, 2003·American Journal of Physiology. Cell Physiology·A P SealeE G Grau
Jan 24, 2003·American Journal of Physiology. Cell Physiology·A P SealeE G Grau
Jun 5, 2003·American Journal of Physiology. Cell Physiology·Atsuko MizunoMakoto Suzuki
Oct 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wolfgang Liedtke, Jeffrey M Friedman
Jan 7, 2004·American Journal of Physiology. Cell Physiology·Bernd NiliusThomas Voets
Apr 27, 2004·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·André P SealeGordon E Grau
May 24, 2005·Pflügers Archiv : European journal of physiology·Roger G O'Neil, Stefan Heller
Mar 8, 2007·The Journal of Biological Chemistry·Joris VriensBernd Nilius
Mar 14, 2007·Experimental Physiology·Wolfgang Liedtke
Dec 18, 2007·Cell·David E Clapham
Jan 5, 2008·The Journal of Biological Chemistry·Dieter D'hoedtBernd Nilius
Oct 2, 2008·The Journal of Biological Chemistry·Anna Garcia-EliasMiguel A Valverde
Dec 26, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Soichi WatanabeToyoji Kaneko
Mar 20, 2009·Molecular Pharmacology·Joris VriensBernd Nilius
Aug 25, 2009·Pflügers Archiv : European journal of physiology·Carole JungMiguel A Valverde
Oct 21, 2009·Cell Biochemistry and Biophysics·Jun Yin, Wolfgang M Kuebler
Sep 11, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Sten OrreniusBoris Zhivotovsky
Nov 9, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jason P BrevesE Gordon Grau
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Valentina BenfenatiMahmood Amiry-Moghaddam
Apr 2, 2011·The Journal of Biological Chemistry·Faisal FectoHan-Xiang Deng
Apr 5, 2011·Cell Calcium·Boris Zhivotovsky, Sten Orrenius
May 13, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel A RyskampDavid Krizaj
May 18, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Maryline BossusCatherine Lorin-Nebel
Nov 1, 2011·General and Comparative Endocrinology·A P SealeE G Grau
Mar 2, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Soichi WatanabeToyoji Kaneko

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Citations

Jan 8, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Maryline BossusCatherine Lorin-Nebel
Sep 18, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mayu InokuchiAndre P Seale
Jan 27, 2015·The Journal of Experimental Biology·Benjamin P MoormanAndre P Seale
Sep 28, 2013·The Journal of Experimental Biology·Alison M GardellDietmar Kültz

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