Differential release of histamine and prostaglandin D2 in rat peritoneal mast cells activated with peptides.

International Archives of Allergy and Applied Immunology
Francesca Levi-Schaffer, M Shalit

Abstract

Rat peritoneal mast cells co-cultured with mouse 3T3 fibroblasts (MC/3T3) are fully responsive to immunologic stimuli. To assess their nonimmunologic activation MC/3T3 were challenged with various peptides. Optimal concentrations of substance P (10(-4) M) and bradykinin (5 x 10(-5) M) induced histamine release of 58.2 +/- 9.3 and 66.8 +/- 6.6%, respectively, while neurotensin (10(-4) M) released only 16.6 +/- 3.7% histamine. Freshly isolated mast cells (F-MC) challenged with the same concentrations of peptides released lower percentages of histamine (substance P 45.6 +/- 5.1%, bradykinin 32.5 +/- 5.3%, neurotensin 11.3 +/- 6.0%). In both MC/3T3 and F-MC, only minute amounts of prostaglandin D2 (PGD2) were produced. In contrast, activation with anti-IgE antibodies and compound 48/80 caused both histamine release and PGD2 generation. Compound 48/80-stimulated MC/3T3 and F-MC released 80.2 +/- 3.4 and 51.8 +/- 6.2% histamine, respectively, and produced 15.4 +/- 2.8 ng/10(6) mast cells and 3.9 +/- 1.4 ng/10(6) mast cells PGD2, respectively. These findings indicate that peptides and bradykinin induce selective release of histamine with no PGD2 production in both F-MC and MC/3T3. Moreover, MC/3T3 preserve their functional characteris...Continue Reading

Citations

Sep 1, 1992·Neurochemical Research·D Johnson, W Krenger
Jan 1, 1994·International Journal of Clinical & Laboratory Research·E Rubinchik, F Levi-Schaffer
Jan 1, 1990·International Journal of Immunopharmacology·M Shalit, F Levi-Schaffer
Jun 18, 2005·Brain Research. Brain Research Reviews·Theoharis C TheoharidesAphrodite Konstantinidou
Dec 31, 2003·Journal of Neuroimmunology·Theoharis C Theoharides, David E Cochrane
Nov 18, 2000·British Journal of Pharmacology·T C TheoharidesA Athanasiou
Mar 14, 1991·The New England Journal of Medicine·J D MorrowL J Roberts
Nov 1, 1994·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F Levi-Schaffer, E Rubinchik
Nov 30, 2002·Journal of Neuroimmunology·Jacques P ZappullaRoland S Liblau
Apr 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M WagenmannR M Naclerio
Aug 26, 2006·Immunology and Allergy Clinics of North America·Ido BacheletYoseph A Mekori
Nov 6, 2003·Microscopy Research and Technique·Gabriella Chieffi BaccariRossella Monteforte
Dec 29, 2006·Annals of the New York Academy of Sciences·Theoharis C Theoharides, Dimitrios Kalogeromitros
May 15, 2007·Immunological Reviews·Theoharis C TheoharidesDimitris Kalogeromitros
May 1, 1990·Experimental Cell Research·F Levi-Schaffer, A Kupietzky
Apr 4, 2006·International Journal of Radiation Oncology, Biology, Physics·Junru WangMartin Hauer-Jensen
Oct 1, 1992·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M ShalitF Levi-Schaffer
Jan 23, 2003·The Journal of Thoracic and Cardiovascular Surgery·Denis SchmartzJean-Louis Vincent
Nov 4, 2015·European Journal of Pharmacology·Roopesh Singh GangwarFrancesca Levi-Schaffer
Dec 28, 2010·Biochimica Et Biophysica Acta·Theoharis C TheoharidesDimitrios Kalogeromitros
Jun 14, 2003·Arquivos de neuro-psiquiatria·Sérgio Luiz Gomes AntunesOlle Johansson
Nov 12, 2005·Journal of Clinical Psychopharmacology·Theoharis C TheoharidesAnthony Clemons
Dec 1, 1991·The Journal of Allergy and Clinical Immunology·M ShalitF Levi-Schaffer
Mar 16, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A BertonF X Maquart
Oct 13, 2006·Journal of Leukocyte Biology·Enrico BaroniDaniele D'Ambrosio
Dec 1, 1990·European Journal of Immunology·F Levi-Schaffer, N Riesel-Yaron
Jul 1, 1992·Infection and Immunity·J KomisarJ Tseng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.