Key features of clathrin-mediated endocytosis have been conserved across evolution. However, endocytosis in Saccharomyces cerevisiae is completely dependent on a functional actin cytoskeleton, whereas actin appears to be less critical in mammalian cell endocytosis. We reveal that the fundamental requirement for actin in the early stages of yeast endocytosis is to provide a strong framework to support the force generation needed to direct the invaginating plasma membrane into the cell against turgor pressure. By providing osmotic support, pressure differences across the plasma membrane were removed and this reduced the requirement for actin-bundling proteins in normal endocytosis. Conversely, increased turgor pressure in specific yeast mutants correlated with a decreased rate of endocytic patch invagination.
High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A
The Ppz protein phosphatases are key regulators of K+ and pH homeostasis: implications for salt tolerance, cell wall integrity and cell cycle progression
Response of the Saccharomyces cerevisiae Mpk1 mitogen-activated protein kinase pathway to increases in internal turgor pressure caused by loss of Ppz protein phosphatases.
Coupling between clathrin-coated-pit invagination, cortactin recruitment, and membrane scission observed in live cells
Interactions between the yeast SM22 homologue Scp1 and actin demonstrate the importance of actin bundling in endocytosis
An actin cytoskeleton with evolutionarily conserved functions in the absence of canonical actin-binding proteins
Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis
Secretory pathway-dependent localization of the Saccharomyces cerevisiae Rho GTPase-activating protein Rgd1p at growth sites
Multicomponent analysis of junctional movements regulated by myosin II isoforms at the epithelial zonula adherens
Fission yeast Sec3 and Exo70 are transported on actin cables and localize the exocyst complex to cell poles
Regulation of cell wall synthesis by the clathrin light chain is essential for viability in Schizosaccharomyces pombe
An Abp1-dependent route of endocytosis functions when the classical endocytic pathway in yeast is inhibited
Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration
Local and global analysis of endocytic patch dynamics in fission yeast using a new "temporal superresolution" realignment method
Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin
Coordinating the cytoskeleton and endocytosis for regulated plasma membrane growth in the early Drosophila embryo
The actin subfamily PtAct4, out of many subfamilies, is differentially localized for specific local functions in Paramecium tetraurelia cells
Dual-colour fluorescence microscopy using yEmCherry-/GFP-tagging of eisosome components Pil1 and Lsp1 in Candida albicans
The role of F-actin in modulating Clathrin-mediated endocytosis: Lessons from neurons in health and neuropsychiatric disorder
Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.