PMID: 9445001Jan 28, 1998Paper

Differential requirements for conserved E2 binding sites in the life cycle of oncogenic human papillomavirus type 31

Journal of Virology
Frank StubenrauchLaimonis A Laimins

Abstract

Human papillomavirus (HPV) E2 proteins regulate viral replication by binding to sites in the upstream regulatory region (URR) and by complex formation with the E1 origin recognition protein. In the genital HPV types, the distribution and location of four E2 binding sites (BS1 to BS4) which flank a single E1 binding site are highly conserved. We have examined the roles of these four E2 sites in the viral life cycle of HPV type 31 (HPV31) by using recently developed methods for the biosynthesis of papillomaviruses from transfected DNA templates (M. G. Frattini et al., Proc. Natl. Acad. Sci. USA 93:3062-3067, 1996). In transient assays, no single site was found to be necessary for replication, and mutation of the early promoter-proximal site (BS4) led to a fourfold increase in replication. Cotransfection of the HPV31 wild-type (HPV-wt) and mutant genomes with expression vectors revealed that E1 stimulated replication of HPV31-wt as well as the HPV31-BS1, -BS2, and -BS3 mutants. In contrast, increased expression of E2 decreased replication of these genomes. Replication of the HPV31-BS4 mutant genome was not further increased by cotransfection of E1 expression vectors but was stimulated by E2 coexpression. In stably transfected norm...Continue Reading

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Citations

May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·A A McBrideC C Baker
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Rashmi S Hegde
Oct 31, 2003·Molecular and Cellular Biology·Thomas ZobelFrank Stubenrauch
Nov 16, 2005·Reviews in Medical Virology·Christy M Hebner, Laimonis A Laimins
Jun 24, 2020·Pathogens·Michelle Mac, Cary A Moody
Nov 26, 2013·Future Microbiology·Shiyuan Hong, Laimonis A Laimins
Sep 5, 2002·Journal of Virology·Jennifer L Bromberg-White, Craig Meyers
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Michelle S Longworth, Laimonis A Laimins
Sep 1, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Melanie BeglinLaimonis Laimins
Oct 16, 2012·International Journal of Cancer. Journal International Du Cancer·Arkom ChaiwongkotMagnus von Knebel Doeberitz
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·J T ThomasL A Laimins
Aug 30, 2008·Journal of Virology·Michael J LaceThomas H Haugen
Jul 2, 2010·Nature Reviews. Cancer·Cary A Moody, Laimonis A Laimins
Apr 11, 2018·Frontiers in Microbiology·Carlotta OliveroGirish K Patel
Jul 10, 2019·Annual Review of Virology·Tami L Coursey, Alison A McBride
Jan 13, 2018·Journal of Investigative and Clinical Dentistry·Gloria I LafaurieDavid Díaz-Báez
Jan 22, 2021·Journal of Virology·Frank StubenrauchRichard B S Roden
Jul 16, 2013·Virology·Alison A McBride
Mar 14, 2000·Seminars in Cancer Biology·F Stubenrauch, L A Laimins

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