PMID: 325516Jan 1, 1977

Differential requirements for polypeptide chain initiation complex formation at the three bacteriophage R17 initiator regions

Nucleic Acids Research
J A SteitzP B Moore

Abstract

The initiation specificity of washed E. coli ribosomes in the presence and absence of purified initiation factors and/or S1 protein has been examined in protection experiments using 32P-labelled R17 RNA. We find that the three bacteriophage initiator regions do not exhibit equal requirements for either of these components during initiation complex formation. Specifically, both factors and S1 stimulate ribosome binding to the beginnings of the coat and replicase cistrons to a greater extent than they promote recognition of the A protein initiation site. The differential effects are therefore inversely correlated with the degree of mRNA-16S rRNA complementarity exhibited by the three initiator regions. We also observe that S1 suppresses ribosome binding to spurious sites in the R17 RNA.

References

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Citations

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D A Steege
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D E DraperP H von Hippel
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C ChangY W Kan
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D E Draper, P H von Hippel
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J R McLaughlinJ C Rabinowitz
Jan 1, 1979·Nucleic Acids Research·P B Moore, M Laughrea
Sep 1, 1967·European Journal of Biochemistry·T Hohn
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J SenguptaJ Frank
Jun 15, 1978·Journal of Molecular Biology·M Laughrea, P B Moore
Aug 25, 1977·Journal of Molecular Biology·J A Steitz, D A Steege
Dec 15, 1981·Journal of Molecular Biology·I Y Sillers, P B Moore
Mar 5, 1991·Journal of Molecular Biology·D HartzL Gold
Dec 15, 1977·Nature·N KennedyP Herrlich

Related Concepts

Bacterial Proteins
Coliphages
Alkalescens-Dispar Group
Cistron
Genetic Code
Peptide Chain Initiation, Translational
Peptide Initiation Factors
Ribosomes
RNA-Directed RNA Polymerase
RNA, Viral

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