Differential response of archaeal and bacterial communities to nitrogen inputs and pH changes in upland pasture rhizosphere soil

Environmental Microbiology
Graeme W NicolJ I Prosser

Abstract

Grassland management regimens influence the structure of archaeal communities in upland pasture soils, which appear to be dominated by as yet uncultivated non-thermophilic Crenarchaeota. In an attempt to determine which grassland management factors select for particular crenarchaeal community structures, soil microcosm experiments were performed examining the effect of increased pH, application of inorganic fertilizer (ammonium nitrate) and sheep urine deposition on both archaeal and bacterial communities in unmanaged grassland soil. As grassland management typically increases pH, a further experiment examined the effect of a reduction in pH, to that typical of unimproved grassland soils, on archaeal and bacterial communities. The RT-PCR amplification of 16S rRNA followed by denaturing gradient gel electrophoresis analysis demonstrated a distinct and reproducible effect on bacterial communities after incubation for 28 or 30 days. In contrast, none of the treatments had a significant effect on the structure of the crenarchaeal community, indicating that these factors are not major drivers of crenarchaeal community structures in grassland soils.

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C R WoeseM L Wheelis
Feb 1, 1997·Applied and Environmental Microbiology·G JurgensA Saano
Jun 11, 1998·Microbial Ecology·A Felske, A D L Akkermans
Jan 23, 1999·Current Opinion in Genetics & Development·E F DeLong
Mar 10, 2001·Environmental Microbiology·H M SimonR M Goodman
Jun 8, 2001·Microbial Ecology·M.K. Chelius, E.W. Triplett
Feb 18, 2003·Environmental Microbiology·Graeme W NicolJames I Prosser
Aug 16, 2003·Environmental Microbiology·Torsten OchsenreiterChrista Schleper
Dec 9, 2003·Applied and Environmental Microbiology·Graeme W NicolJames I Prosser

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Citations

Nov 19, 2010·The ISME Journal·Scott T BatesNoah Fierer
Jun 3, 2005·Nature Reviews. Microbiology·Christa SchleperMelanie Jonuscheit
Aug 5, 2008·Applied and Environmental Microbiology·Chuanlun L ZhangBrian P Hedlund
Jan 4, 2013·PloS One·Michele C Pereira e SilvaJoana Falcão Salles
Mar 28, 2013·Archaea : an International Microbiological Journal·Elisabeth W VissersHendrikus J Laanbroek
Aug 6, 2008·The Science of the Total Environment·Malcolm S CresserIshaq A Mian
Feb 10, 2007·Journal of Microbiological Methods·Andrzej Brunon PoplawskiUlla Rasmussen
May 17, 2005·Environmental Microbiology·J Ignacio Rangel-CastroJim I Prosser
Feb 3, 2005·Environmental Microbiology·Graeme W NicolJames I Prosser
May 13, 2006·FEMS Microbiology Ecology·Shahid Mahmood, James I Prosser
Sep 8, 2009·FEMS Microbiology Ecology·Laura E LehtovirtaGraeme W Nicol
Jan 10, 2012·FEMS Microbiology Ecology·Peng CaoJi-Zheng He
Jul 27, 2007·Critical Reviews in Microbiology·Elena V PikutaJane Tang

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