PMID: 2507758Aug 1, 1989Paper

Differential response of excision proficient and deficient L5178Y cells to UVC irradiation and benzamide

Journal of Photochemistry and Photobiology. B, Biology
I SzumielK J Johanson

Abstract

L5178Y-R and L5178Y-S cells differ in sensitivity to UVC radiation (D0 values: 2.8 and 9.0 J m-2 respectively, exposure in Fischer's medium). The UVC sensitivity is related to the excision repair ability. Benzamide (Bz), an inhibitor of adenosine diphosphoribosyl transferase (ADPRT), does not modify the lethal effect of UVC radiation in L5178Y-R cells, whereas it sensitizes L5178Y-S cells. The content of NAD+ after irradiation decreases only in the latter cells and this decrease can be prevented by 2 mM Bz treatment. In agreement with the survival data, in L5178Y-R cells neither the proportion of abnormal cells nor the frequency of chromatid aberration are affected by 2 mM Bz treatment, in contrast with L5178Y-S cells. Bz slightly reverses inhibition of 3H-thymidine incorporation only in L5178Y-S cells, but it does not affect the proportions of cells in the different phases of the cell cycle in either cell strain after UVC exposure. These data could be taken as an indirect indication of the involvement of ADPRT in DNA repair in UVC-irradiated L5178Y-S cells. However, the increase in the number of DNA strand breaks in UVC-exposed, Bz-treated cells compared with UVC-exposed untreated cells is the same in both L5178Y strains.

References

Aug 1, 1976·Archives of Biochemistry and Biophysics·E L Jacobson, M K Jacobson
Dec 11, 1978·Biochimica Et Biophysica Acta·J E Cleaver
Jan 1, 1987·Journal of Cell Science. Supplement·L H ThompsonC A Weber
May 1, 1985·Somatic Cell and Molecular Genetics·P G McKennaV J McKelvey
Sep 1, 1982·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·E BocianB Ziemba-Zółtowska

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Citations

Jul 1, 1990·Cell Biology and Toxicology·H E KleczkowskaF R Althaus
Jan 1, 1991·Radiation and Environmental Biophysics·S Hillebrandt, I Müller
Aug 4, 2005·International Journal of Radiation Biology·I Szumiel

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