PMID: 2493458Mar 1, 1989Paper

Differential response of myofibrillar and cytoskeletal proteins in cells treated with phorbol myristate acetate

The Journal of Cell Biology
Z LinH Holtzer

Abstract

Muscle-specific and nonmuscle contractile protein isoforms responded in opposite ways to 12-o-tetradecanoyl phorbol-13-acetate (TPA). Loss of Z band density was observed in day-4-5 cultured chick myotubes after 2 h in the phorbol ester, TPA. By 5-10 h, most I-Z-I complexes were selectively deleted from the myofibril, although the A bands remained intact and longitudinally aligned. The deletion of I-Z-I complexes was inversely related to the appearance of numerous cortical, alpha-actinin containing bodies (CABs), transitory structures approximately 3.0 microns in diameter. Each CAB consisted of a filamentous core that costained with antibodies to alpha-actin and sarcomeric alpha-actinin. In turn each CAB was encaged by a discontinuous rim that costained with antibodies to vinculin and talin. Vimentin and desmin intermediate filaments and most cell organelles were excluded from the membrane-free CABs. These curious bodies disappeared over the next 10 h so that in 30-h myosacs all alpha-actin and sarcomeric alpha-actinin structures had been eliminated. On the other hand vinculin and talin adhesion plaques remained prominent even in 72-h myosacs. Disruption of the A bands was first initiated after 15-20 h in TPA (e.g., 15-20-h myos...Continue Reading

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Citations

Jan 1, 1990·Cell Motility and the Cytoskeleton·J J Otto
Jul 9, 1999·Reviews of Physiology, Biochemistry and Pharmacology·M GautelP Young
Jul 9, 1999·Reviews of Physiology, Biochemistry and Pharmacology·A B Fulton
Dec 4, 2003·Experimental Cell Research·Monique BerendseCatriona M Lloyd
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Jul 12, 2014·European Journal of Cell Biology·Krzysztof M BernadzkiTomasz J Prószyński
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Oct 19, 1995·Biochimica Et Biophysica Acta·A HattoriR Tatsumi
Aug 20, 2021·Journal of Muscle Research and Cell Motility·Geyse GomesManoel Luis Costa

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