Differential response of neural cells to trauma-induced free radical production in vitro

Neurochemical Research
K S PanickarMichael D Norenberg

Abstract

CNS trauma has been associated with an increase in free radical production, but the cellular sources of this increase or the mechanism involved in the production of free radicals are not known. We, therefore, investigated the effects of trauma on free radical production in cultured neurons, astrocytes and BV-2 microglial cells. Free radicals were measured with the fluorescent dye DCFDA following in vitro trauma. At 30 and 60 min following trauma, there was a 132% and 64% increase, respectively, in free radical production in neurons when compared to controls. In astrocytes, there was a 94% and 133% increase at 30 and 60 min, respectively. Microglial cells, however, displayed no significant increase in free radicals at 30, 60 or 120 min following trauma. Since trauma can induce the mitochondrial permeability transition (MPT), a process associated with mitochondrial dysfunction, we further investigated whether cyclosporin A (CsA), an agent known to block the MPT, could prevent free radical formation following trauma. In neurons CsA did not block free radical production at 30 min but blocked it by 90% at 60 min. In contrast, in astrocytes CsA completely blocked free radical production at 30 min but did not block it at 60 min. Our r...Continue Reading

Citations

Jan 6, 2011·Journal of Neurotrauma·Kakulavarapu V Rama RaoMichael D Norenberg
Jan 30, 2014·Journal of Neurotrauma·Arumugam R JayakumarMichael D Norenberg
Oct 11, 2017·Alternatives to Laboratory Animals : ATLA·Ashwin Kumaria
Nov 19, 2017·Neurochemical Research·A R JayakumarM D Norenberg
May 3, 2011·Annual Review of Biomedical Engineering·Barclay MorrisonMartin L Yarmush
Apr 1, 2009·Journal of Neurotrauma·Susan MarguliesUNKNOWN Combination Therapies for Traumatic Brain Injury Workshop Leaders
Dec 27, 2008·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Robert Vink, Alan J Nimmo
Feb 22, 2012·Biological Research for Nursing·Karen M O'Connell, Marguerite T Littleton-Kearney
Apr 24, 2008·Journal of Neuropathology and Experimental Neurology·Arumugam R JayakumarMichael D Norenberg
Sep 15, 2012·Transplant International : Official Journal of the European Society for Organ Transplantation·Meirav KedmiMichael Y Shapira
Mar 14, 2020·Neurochemical Research·Mehran TaherianArumugam R Jayakumar
Apr 3, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Edgar A JaimesLeopoldo Raij
Feb 11, 2011·Journal of Neurochemistry·Arumugam R JayakumarMichael D Norenberg
Oct 6, 2004·Expert Opinion on Investigational Drugs·Robert Vink, Corinna Van Den Heuvel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.