Differential response to EGFR- and VEGF-targeted therapies in patient-derived tumor tissue xenograft models of colon carcinoma and related metastases

International Journal of Oncology
Ketao JinLisong Teng

Abstract

Heterogeneity in primary tumors and related metastases may result in failure of antitumor therapies, particularly in targeted therapies for the treatment of cancer. In this study, patient-derived tumor tissue (PDTT) xenograft models of colon carcinoma with lymphatic and hepatic metastases were used to evaluate the response to EGFR- and VEGF-targeted therapies. Our results showed that primary colon carcinoma and its corresponding lymphatic and hepatic metastases have a different response rate to anti-EGFR (cetuximab) and anti-VEGF (bevacizumab) therapies. However, the underlying mechanism of these types of phenomenon is still unclear. To investigate whether such phenomena may result from the heterogeneity in primary colon carcinoma and related metastases, we compared the expression levels of cell signaling pathway proteins using immunohistochemical staining and western blotting, and the gene status of KRAS using pyrosequencing in the same primary colon carcinoma and its corresponding lymphatic and hepatic metastatic tissues which were used for establishing the PDTT xenograft models. Our results showed that the expression levels of EGFR, VEGF, Akt/pAkt, ERK/pERK, MAPK/pMAPK, and mTOR/pmTOR were different in primary colon carcinom...Continue Reading

References

Jan 15, 1997·Journal of the National Cancer Institute·G GaspariniT Tominaga
Mar 29, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·B LinderholmR Henriksson
Aug 15, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·D GancbergD Larsimont
Mar 3, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Leonard B SaltzRobert J Mayer
Mar 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gottfried E KonecnyMark D Pegram
Jul 20, 2004·The Journal of Pathology·Peter RegitnigSigurd F Lax
Jul 23, 2004·The New England Journal of Medicine·David CunninghamEric Van Cutsem
Jul 29, 2005·The Journal of Molecular Diagnostics : JMD·Shuji OginoCharles S Fuchs
Aug 11, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Belen Rubio-ViqueiraManuel Hidalgo
May 22, 2007·Breast Cancer Research : BCR·Coya TapiaLukas Bubendorf
Mar 5, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Rafael G AmadoDavid D Chang
Apr 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Julie M WuPedram Argani
Jun 26, 2008·British Journal of Cancer·F CappuzzoP A Jänne
Jun 3, 2009·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Sarah ParkMyung-Ju Ahn
Jan 28, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Stephan E BaldusHelmut E Gabbert
Jul 10, 2010·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Ketao JinGuangliang Li
Jul 13, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·C F VerschraegenT Bocklage
Mar 1, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·C F VerschraegenT Bocklage

❮ Previous
Next ❯

Citations

Oct 26, 2013·Cancer Letters·Prerna MalaneyVrushank Davé
May 14, 2017·Journal of Hematology & Oncology·Yunxin LaiPeng Li
Feb 20, 2015·Journal of the National Cancer Institute·Angela Broggini-TenzerMartin Pruschy
Sep 25, 2015·Pharmacogenomics·Daniela SiaAugusto Villanueva
Dec 21, 2013·Cancer Discovery·Lidia MoserleOriol Casanovas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.