PMID: 6165989Jan 1, 1981Paper

Differential response to growth factor by rat mammary epithelium plated on different collagen substrata in serum-free medium

Proceedings of the National Academy of Sciences of the United States of America
D S SalomonW R Kidwell

Abstract

Primary cultures of rat mammary epithelial cells proliferate and synthesize basement membrane collagen (type IV collagen) in a serum-free medium supplemented with epidermal growth factor (EGF), hydrocortisone or dexamethasone, insulin, transferrin, and Pedersen fetuin. The growth response of the cells to EGF and glucocorticoids but not to insulin or transferrin varies depending on the substratum on which the cells are plated. Cell growth is 4 times more sensitive to omission of EGF or glucocorticoid on type I collagen or plastic substratum than on type IV collagen substratum. The mechanism by which these two growth factors differentially affect cell growth appears to be linked to an increase in type IV collagen synthesis and a stabilization of secreted type IV collagen in the extracellular matrix. Glucocorticoids suppress the elaboration of type IV collagenolytic activity by the cells whereas EGF stimulates amino acid incorporation into type IV collagen. The results suggest that EGF and glucocorticoids affect mammary epithelial cell growth by facilitating the accumulation of the appropriate cell substratum.

References

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L A LiottaG R Martin
Dec 15, 1977·International Journal of Cancer. Journal International Du Cancer·J Taylor-PapadimitriouM G Stoker
Jan 1, 1979·Annual Review of Biochemistry·G Carpenter, S Cohen
Nov 1, 1979·Experimental Cell Research·M S WichaW R Kidwell
Mar 1, 1978·European Journal of Biochemistry·R TimplH Wiedemann
Jun 1, 1978·Nature·J Folkman, A Moscona
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K J Lembach
Oct 1, 1971·Biochemical and Biophysical Research Communications·N A Kefalides
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Gospodarowicz, C R Ill
Mar 1, 1980·Analytical Biochemistry·D Barnes, G Sato

❮ Previous
Next ❯

Citations

Jul 1, 1990·Cancer Metastasis Reviews·F R Miller, G H Heppner
Apr 1, 1992·Cell and Tissue Research·J E FergusonM W Ferguson
Apr 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·Y TomookaJ A McLachlan
May 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A GaugerM A Riederer-Henderson
Oct 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D BarnesG H Sato
May 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D E IngberJ Folkman
Aug 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S P Ethier
Aug 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H A Hahm, M M Ip
Aug 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H A HahmT Oka
Jan 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D DanielpourD A Sirbasku
Jan 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B SanfilippoW R Kidwell
Aug 1, 1997·Archives of Pharmacal Research·D Y KimN D Kim
May 7, 2008·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Eberhard StennertAxel Mickenhagen
Jun 25, 1998·In Vitro Cellular & Developmental Biology. Animal·R C HoveyT B McFadden
Jan 1, 1982·Tissue & Cell·D D MackenzieA Turvey
Dec 17, 1985·Biochimica Et Biophysica Acta·A E Lagarde, R S Kerbel
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Taub
Sep 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·A C CatoP Herrlich
Jan 1, 1985·Collagen and Related Research·M J Barnes
Oct 20, 1998·Histopathology·P P OsinB A Gusterson
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M S WichaT Mahn
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W ImagawaS Nandi
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H G HallM J Bissell
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S D LuikartA C Sartorelli
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V P TerranovaL A Liotta
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P EkblomR Timpl
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R A MajackP Bornstein
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I VlodavskyM Klagsbrun
May 1, 1990·The Journal of Cell Biology·D E IngberM A Schwartz
Sep 1, 1982·The Journal of Cell Biology·K AlitaloA Vaheri
Mar 1, 1984·The Journal of Cell Biology·U K EhmannD S Misfeldt
Sep 1, 1987·Annals of Surgery·J Folkman, D E Ingber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.