Differential response to hepatic differentiation stimuli of amniotic epithelial cells isolated from four regions of the amniotic membrane.

Journal of Cellular and Molecular Medicine
Francesca PassarettaRoberta Di Pietro

Abstract

Human Amniotic Epithelial Cells (hAEC) isolated from term placenta are a promising source for regenerative medicine. However, it has long been debated whether the hAEC population consists of heterogeneous or homogeneous cells. In a previous study, we investigated the characteristics of hAEC isolated from four different regions of the amniotic membrane finding significant heterogeneity. The aim of this study was to evaluate the hepatic differentiation capability of hAEC isolated from these four regions. Human term placentae were collected after caesarean section and hAEC were isolated from four regions of the amniotic membrane (R1-R4, according to their relative distance from the umbilical cord) and treated in hepatic differentiation conditions for 14 days. hAEC-derived hepatocyte-like cells showed marked differences in the expression of hepatic markers: R4 showed higher levels of Albumin and Hepatocyte Nuclear Factor (HNF) 4α whereas R1 expressed higher Cytochrome P450 enzymes, both at the gene and protein level. These preliminary results suggest that hAEC isolated from R1 and R4 of the amniotic membrane are more prone to hepatic differentiation. Therefore, the use of hAEC from a specific region of the amniotic membrane should ...Continue Reading

References

Mar 3, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jeanine L PageCurtis J Omiecinski
May 4, 2010·Veterinary Research Communications·A MauroP Berardinelli
Mar 5, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Fabio MarongiuStephen C Strom
Jul 14, 2012·Journal of Internal Medicine·C JornsB G Ericzon
Dec 1, 2013·Stem Cells and Development·Stephen C StromToshio Miki
Mar 23, 2018·Cell Transplantation·Lucia CenturioneRoberta Di Pietro

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Citations

Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Adelheid WeidingerAsmita Banerjee

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Methods Mentioned

BETA
electron microscopy

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