Differential responses in the biotransformation systems of the oyster Crassostrea gigas (Thunberg, 1789) elicited by pyrene and fluorene: Molecular, biochemical and histological approach - Part II

Aquatic Toxicology
Isis Mayna Martins Dos ReisAfonso Celso Dias Bainy

Abstract

Pyrene (PYR) and fluorene (FLU) are among the sixteen priority Polycyclic Aromatic Hydrocarbons (PAH) of the United States Environmental Protection Agency and are both frequently detected in contaminated sites. Due to the importance of bivalve mollusks in biomonitoring programs and the scarce information on the biotransformation system in these organisms, the aim of this study was to investigate the effect of PYR and FLU at the transcriptional level and the enzymatic activities of some biotransformation systems in the Pacific oyster Crassostrea gigas, and to evaluate the histological effects in their soft tissues. Oysters C. gigas were exposed for 24 h and 96 h to PYR (0.25 and 0.5 μM) and FLU (0.6 and 1.2 μM). After exposure, transcript levels of cytochrome P450 coding genes (CYP1-like, CYP2-like, CYP2AU2, CYP356A1, CYP17α-like), glutathione S tranferase genes (omega GSTO-like and microsomal, MGST-like) and sulfotransferase gene (SULT-like), and the activity of ethoxyresorufin O-deethylase (EROD), Glutathione S-transferase (GST) and microssomal GST (MGST) were evaluated in gills. Histologic changes were also evaluated after the exposure period. PYR and FLU bioconcentrated in oyster soft tissues. The half-life time of PYR in wa...Continue Reading

References

Jan 1, 1989·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·D Schlenk, D R Buhler
Apr 28, 2000·The Journal of Biological Chemistry·P G BoardJ Pandit
Aug 24, 2000·Critical Reviews in Toxicology·J J WhyteD E Tillitt
Jul 9, 2002·Environmental Science & Technology·Francesco CastelliMaria Grazia Sarpietro
Sep 6, 2002·Chemico-biological Interactions·Mark E Hahn
Apr 15, 2004·Toxicology and Applied Pharmacology·John P IncardonaNathaniel L Scholz
Nov 24, 2004·Environmental Toxicology and Chemistry·Aaron J RustAnne E McElroy
Jun 28, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Montserrat Solé, David R Livingstone
Dec 3, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Niranjali GamageMichael E McManus
Feb 14, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Maciej J Zamek-GliszczynskiKim L R Brouwer
Feb 18, 2006·Environment International·Oya S OkayPeter Donkin
Sep 16, 2006·Aquatic Toxicology·Yoshinori IkenakaYuichi Miyabara
Nov 28, 2006·Nature Reviews. Cancer·Daniel W Nebert, Timothy P Dalton
Jan 24, 2007·Biochimica Et Biophysica Acta·Emre M Isin, F Peter Guengerich
Feb 20, 2007·Biochemical and Biophysical Research Communications·Atchasai SiritantikornRalf Morgenstern
Jul 31, 2007·Environmental Research·F X Valdez DomingosC A Oliveira Ribeiro
Sep 29, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cora BurmeisterEva Liebau
Feb 19, 2008·Ecotoxicology and Environmental Safety·Yuanyuan SunYuqun Xue
Apr 9, 2008·Marine Environmental Research·Guilherme de Toledo-SilvaAfonso C D Bainy
Apr 9, 2008·Marine Environmental Research·Igor Dias MedeirosAfonso Celso Dias Bainy
May 2, 2008·Micron : the International Research and Review Journal for Microscopy·José Augusto de Oliveira DavidCarmem S Fontanetti
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Sep 5, 2008·Environmental Research·Renata GonçalvesAna D Correia
Nov 27, 2008·Bulletin of Environmental Contamination and Toxicology·G H LuZ Zhu
Jun 26, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniel G BeachJocelyne Hellou
Dec 8, 2009·Marine Environmental Research·Jaco J MattosAfonso C D Bainy
Aug 24, 2010·Free Radical Biology & Medicine·Katarina JohanssonRalf Morgenstern
Nov 10, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Curtis J OmiecinskiJeffrey M Peters
Dec 28, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Denise FernandesCinta Porte
Mar 31, 2011·International Journal for Parasitology·Benjamin MorgaIsabelle Arzul
Jun 1, 2003·Environmental Toxicology and Pharmacology·Vito LibrandoFrancesco Castelli
Nov 1, 2008·Environmental Toxicology and Pharmacology·Igor D MedeirosAfonso C D Bainy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.