Abstract
Hypoxia, an endocrine disruptor, affects synthesis and balance of sex steroid hormones, leading to reproductive impairment in both female and male fish. Cumulating reports demonstrated the alternation of hypothalamus-pituitary-gonad axis (HPG-axis) by hypoxia. However, the detail mechanism underlying how hypoxia may alter other brain functions remains largely unknown. In this report, we used marine medaka as a model and conducted a high-throughput RNA sequencing followed by bioinformatics analysis on hypoxia-exposed brain tissues, aiming to determine the change of transcriptional signature and to unravel the pathways that are induced by hypoxia. We found that hypoxia lead to dysregulation of brain functions (including synaptic transmission, axon guidance, potassium ion transport, neuron differentiation, and development of brain and pituitary gland), and also signaling pathways (e.g., gap junction, calcium signaling pathway, and GnRH signaling pathway). Our results further demonstrate gender-specific responses to hypoxia in female and male fish's brains, which provides novel insights into the mechanism underlying the hypoxia induced sex specific brain functions impairments.
References
Mar 1, 1991·Neuron·Z G XiaD R Storm
Mar 30, 1995·Nature·D M CooperJ W Karpen
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z L WuD R Storm
May 1, 1996·Progress in Neurobiology·C NyakasP G Luiten
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·E T TzavaraJ Hanoune
Aug 11, 1998·Neurotoxicology and Teratology·M E Gilbert, D Liang
May 3, 2001·Environmental Health Perspectives·A C Melo, J S Ramsdell
Jul 14, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·L G ParksL E Gray
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Juliane StieberAndreas Ludwig
Jul 26, 2005·Nature Genetics·Jernej UleRobert B Darnell
Jan 6, 2006·Environmental Health Perspectives·Jennifer A FentressAnn Oliver Cheek
May 25, 2006·Environmental Science & Technology·Eva H H ShangRudolf S S Wu
Jun 13, 2006·Marine Environmental Research·Peter ThomasSusan Lawson
Dec 1, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lisa M HinesUNKNOWN World Health Organization/International Society for Biomedical Research on Alcoholism Study on State and Trait Markers of Al
Aug 30, 2007·Proceedings. Biological Sciences·Peter ThomasJames A Kummer
May 26, 2009·Nucleic Acids Research·Ruben Nogales-CadenasAlberto Pascual-Montano
Jul 3, 2009·Environmental Science & Technology·Peter Thomas, Md Saydur Rahman
Nov 17, 2009·Bioinformatics·Mark D RobinsonGordon K Smyth
Apr 13, 2011·Biological Psychiatry·Sylvane DesrivièresBoris Tabakoff
Mar 16, 2012·Bioinformatics·Jing-Woei LiDavid Mittelman
May 11, 2012·Nucleic Acids Research·Daniel Tabas-MadridAlberto Pascual-Montano
Apr 25, 2013·Brain Research·Jonathan ChenLonny R Levin
Jan 1, 2014·Genomics·Jing-Woei LiTing-Fung Chan
Mar 7, 2014·PloS One·Jessica TiedkeThorsten Burmester
Apr 17, 2014·Chemical Society Reviews·Tuhin PradhanJong Seung Kim
Oct 29, 2014·PloS One·Karen LauRudolf Shiu Sun Wu
Nov 9, 2014·Cancer Research·Nicole M WarringtonJoshua B Rubin
Mar 15, 2015·BMC Genomics·Keng Po LaiRudolf Shiu-Sun Wu
Apr 25, 2015·Scientific Reports·Jie GuChris Kong-Chu Wong
Jun 25, 2015·The EMBO Journal·Peter H StörchelGerhard Schratt