Differential responses of granulosa cells from small and large follicles to follicle stimulating hormone (FSH) during the menstrual cycle and acyclicity: effects of tumour necrosis factor-alpha

Human Reproduction
V Montgomery RiceP F Terranova

Abstract

This study determined effects of follicle stimulating hormone (FSH) alone and in combination with tumour necrosis factor (TNF), on granulosa cells from small (5-10 mm diameter) and large (>10-25 mm) follicles during follicular and luteal phases of the cycle and during periods of acyclicity. Granulosa cells were collected from ovaries of premenopausal women undergoing oophorectomy. The cells were cultured with human FSH (2 ng/ml) and testosterone (1 microM) in the presence or absence of human TNF-alpha (20 ng/ml). Media were removed at 48 and 96 h after culture and progesterone, oestradiol and cAMP in media were measured by radioimmunoassays. FSH stimulated the accumulation of oestradiol from granulosa cells of small follicles during the follicular and luteal phases but not during acyclicity; and TNF reduced oestradiol accumulation in the presence of FSH. Interestingly, in granulosa cells from small follicles, progesterone and cAMP secretion increased in response to FSH and neither was affected by TNF. Thus, TNF specifically inhibited the conversion of testosterone to oestradiol in granulosa cells from small follicles. FSH stimulated oestradiol production by granulosa cells of large follicles obtained only during the follicular ...Continue Reading

Citations

Aug 27, 2013·Journal of Assisted Reproduction and Genetics·M L N DeepikaParveen Jahan
Feb 13, 2001·Molecular and Cellular Endocrinology·S GhersevichP Vihko
Oct 1, 2014·Human Reproduction·E ScaliciS Hamamah
Aug 19, 2007·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Flor Gonzalez-NavarreteLuigi Devoto
Mar 5, 2011·Crisis·Saška RoškarAndrej Marušič
Nov 5, 2016·Metabolic Syndrome and Related Disorders·Josiane Bazzo de AlencarAna Maria Sell
Feb 24, 2015·Reproductive Sciences·Alessandra Aparecida VirequeAna Carolina Japur de Sá Rosa-e-Silva

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