Differential responses of pulmonary endothelial phenotypes to cyclical stretch

Microvascular Research
Jarrod B AdkisonJames C Parker

Abstract

Endothelial phenotypes derived from different pulmonary vascular segments have markedly different permeability response to inflammatory agonists, but their responses to mechanical strain have not been characterized. Therefore, we evaluated the effect of cyclical stretch on cell shape, cell membrane wounding, and junctional beta-catenin in rat pulmonary artery (RPAEC) and microvascular (RPMVEC) endothelial cell monolayers. After 24 h of 24% uniaxial strain at 40 cycles/min, RPAEC but not RPMVEC reoriented transverse to the axis of strain. Total beta-catenin increased in RPAEC but decreased in RPMVEC. Transient plasma membrane wounding was produced by cyclical biaxial strain of 34% or by scratching of monolayers with a needle and was indicated by retention of lysine fixable fluorescent 70 kDa dextran. Junctional beta-catenin was quantified by fluorescence intensity and image analysis. beta-catenin fluorescence was significantly lower in wounded cells than in adjacent uninjured cells in both phenotypes, and the decrease was significantly greater in RPAEC compared to RPMVEC in both scratched (57% vs. 30%) and stretched (55% vs. 37%) cells. Using immunoprecipitation, VE-cadherin-associated beta-catenin decreased significantly in RPA...Continue Reading

References

Dec 29, 1995·The Journal of Biological Chemistry·P NavarroE Dejana
Apr 1, 1996·The Journal of Physiology·C R Neal, C C Michel
Jan 1, 1996·Seminars in Thrombosis and Hemostasis·J G GarciaK L Schaphorst
May 1, 1997·American Journal of Respiratory Cell and Molecular Biology·D E deMelloL M Reid
Nov 18, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J M Daniel, A B Reynolds
Feb 28, 1998·The Journal of Cell Biology·T MollD Vestweber
Jan 6, 2001·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·S Y Yuan
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Kenneth J Cavanaugh, Susan S Margulies
Oct 22, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·J C Parker, S Yoshikawa
Oct 31, 2002·American Journal of Respiratory and Critical Care Medicine·Nicholas E VlahakisRolf D Hubmayr
Dec 14, 2002·American Journal of Respiratory and Critical Care Medicine·Ognjen GajicRolf D Hubmayr
Jan 28, 2003·Current Opinion in Critical Care·Nicholas E Vlahakis, Rolf D Hubmayr
Mar 18, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Konstantin G BirukovJoe G N Garcia
May 16, 2003·Vascular Pharmacology·Sarah Y Yuan
May 22, 2003·The American Journal of Pathology·John K McGuireWilliam C Parks
May 27, 2003·Cell Calcium·Donna L CioffiTroy Stevens
Oct 28, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Diego F AlvarezMary I Townsley
Nov 18, 2003·The Journal of Clinical Investigation·Zeenat SafdarJahar Bhattacharya
Jun 19, 2004·Endothelium : Journal of Endothelial Cell Research·John D KakisisBauer E Sumpio
Jun 19, 2004·Endothelium : Journal of Endothelial Cell Research·Yi-Jen ChiuKeigi Fujiwara
Jun 29, 2004·Microvascular Research·Sarah Gebb, Troy Stevens
Jul 23, 2004·Physiological Reviews·Gianfranco Bazzoni, Elisabetta Dejana
Feb 8, 2005·American Journal of Respiratory and Critical Care Medicine·Nicholas E Vlahakis, Rolf D Hubmayr
Jun 9, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·James C ParkerJudy A King

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Citations

Apr 12, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sarah L SaynerTroy Stevens
Jun 21, 2013·International Journal of Molecular Medicine·Yuxi WangYanyan Gao
Sep 13, 2019·American Journal of Respiratory Cell and Molecular Biology·Kolene E BaileyChelsea M Magin
Feb 2, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Karthik SureshLarissa A Shimoda

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