Differential responsiveness of dopamine-beta-hydroxylase gene expression to glucoprivation in different catecholamine cell groups

Endocrinology
Ai-Jun LiSue Ritter

Abstract

Hindbrain catecholaminergic neurons are key participants in systemic glucoregulation. However, the specific subpopulations critical for glucoregulatory function have not been fully identified. Here we used in situ hybridization and immunohistochemistry to investigate effects of glucoprivation on expression of the gene for the catecholamine biosynthetic enzyme, dopamine-beta-hydroxylase (DBH), to further localize the critical cell populations. Glucoprivation induced by the glycolytic inhibitor, 2-deoxy-D-glucose (2DG) (250 mg/kg) increased total DBH mRNA expression in caudal ventrolateral medullary cell groups (namely A1, the A1/C1 overlap, and the middle portion of C1) from six to 49 times control levels. In retrofacial C1, no enhancement was observed. In the dorsomedial medulla, hybridization signal was modestly increased (tripled) in A2 but was not increased in the area postrema. Previous microinjection of the retrogradely transported catecholamine immunotoxin (anti-DBH-saporin, or DSAP) into the paraventricular nucleus of the hypothalamus reduced the number of DBH-immunoreactive cells in cell groups known to project to the paraventricular nucleus of the hypothalamus as well as reducing the 2DG-stimulated increases in total D...Continue Reading

Citations

Apr 12, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ai-Jun LiSue Ritter
Sep 2, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M F WiaterS Ritter
Jan 9, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ai-Jun LiSue Ritter
Mar 25, 2009·FEBS Letters·Bei ZhouFumiaki Uchiumi
Jul 5, 2008·Brain Research Bulletin·Débora Simões Almeida ColombariSergio Luiz Cravo
Feb 10, 2007·Physiology & Behavior·Kelli TaylorSue Ritter
Jul 2, 2008·Annals of the New York Academy of Sciences·Keiichi Itoi
Mar 10, 2010·Journal of Neuroendocrinology·K Itoi, N Sugimoto
Aug 5, 2006·Physiology & Behavior·Sue RitterAi-Jun Li
Oct 26, 2010·Brain Research·Bruce M KingGeorge A Bray
Sep 17, 2015·PloS One·Megan E GarlapowTrudy F C Mackay
Jan 2, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ai-Jun LiSue Ritter
Jun 13, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ai-Jun LiSue Ritter
Apr 4, 2009·Physiological Reviews·Richard KvetnanskyMiklos Palkovits
Sep 29, 2020·Pharmacology & Therapeutics·Patricia Seoane-CollazoMiguel López
Jan 17, 2021·International Journal of Molecular Sciences·Karen P BriskiAyed A Alshamrani

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