PMID: 7528245Jan 1, 1995Paper

Differential responsiveness of Langerhans cell subsets of varying phenotypic states in normal human epidermis

The Journal of Investigative Dermatology
A ShibakiK D Cooper

Abstract

Epidermal Langerhans cell heterogeneity is poorly understood with regard to phenotypic characteristics, such as the expression of human leukocyte antigen (HLA)-DR, integrin, and Fc receptor molecules, as well as functional characteristics, such as the ability to process and present antigens or produce cytokines during various phases of immigration and maturation. Technical limitations of Langerhans cell number have limited functional assays on putative Langerhans cell subsets in in vivo epidermis. Therefore, we used flow cytometry for simultaneous phenotypic and functional assessment at the single-cell level within the Langerhans cell population. Freshly isolated human epidermal cell suspensions were stained with a battery of monoclonal antibodies, including anti-HLA-DR, -CD1a, -CD1c, -CD11c, -Fc gamma RII, and -Fc epsilon RI. Two distinct Langerhans cell subsets were identified by their different levels of HLA-DR expression. The DRHi subset expressed higher amounts of CD11c and exhibited greater cytoplasmic complexity and higher baseline calcium than the DRLo subset (p < or = 0.03 for each). Some subjects also expressed high levels of Fc epsilon RI in the DRHi, CD11cHi subset. To determine whether these phenotypic subsets may ...Continue Reading

Citations

Sep 1, 1999·The British Journal of Dermatology·M CumberbatchI Kimber
Mar 29, 2000·The British Journal of Dermatology·I KimberC E Griffiths
Jun 25, 2004·The British Journal of Dermatology·M BhushanC E M Griffiths
Jun 26, 2004·Experimental Dermatology·Rebecca J DearmanChristopher E M Griffiths
Jan 13, 2005·The Journal of Veterinary Medical Science·Makoto BonkobaraKiyoshi Ariizumi
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jagadeesh BayrySrini V Kaveri
Apr 18, 2003·Clinical and Experimental Immunology·M CumberbatchC E M Griffiths

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