PMID: 8581996Oct 1, 1995Paper

Differential role of protein tyrosine phosphorylation/dephosphorylation in affinity regulation of beta 1 and beta 3 integrin in human fibroblasts

Cell Structure and Function
J Takagi, Y Saito

Abstract

We investigated the effects of protein tyrosine phosphatase inhibitors, pervanadate and phenylarsine oxide (PAO), on the beta 1 and beta 3 integrin-mediated cell-substrate interaction using normal diploid human fibroblast. Pervanadate treatment of the cells in suspension state resulted in highly elevated levels of cellular protein tyrosine phosphorylation accompanied by loss of beta 1 integrin-mediated adhesion to substrata (i.e., collagen and laminin). In contrast, beta 3 integrin-mediated adhesion to substrata (i.e., fibronectin and vitronectin) of these cells was less affected. Moreover, pervanadate could reverse beta 1 integrin-dependent adhesion, and cells already adhered on collagen or laminin, but not on fibronectin or vitronectin, came off within 30 min upon pervanadate treatment. These effects are likely to be directly mediated by increased cellular protein tyrosine phosphorylation, because another chemical compound, PAO, which also inhibits protein tyrosine phosphatase through a quite different mechanism, also exhibited the specific deterioration of beta 1 integrin-mediated cell-substrate interaction. Upon treatment with these protein tyrosine phosphatase inhibitors, the well developed actin stress fibers were disrupt...Continue Reading

Citations

Oct 27, 2005·Journal of Cellular Physiology·Maria Teresa Herrera AbreuGregory P Downey
Jul 6, 2010·Molecular Reproduction and Development·Venkatesh KotaSisinthy Shivaji
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H E MathenyJ M Cook-Mills

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