differential roles for the E2A activation domains in B lymphocytes and macrophages.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Savita BhallaBarbara L Kee

Abstract

The E2A gene encodes two E protein/class I basic helix-loop-helix transcription factors, E12 and E47, that are essential for B lymphopoiesis. In addition to the DNA-binding and protein dimerization domain, the E proteins share two highly conserved transcription activation domains. In this study, we show that both activation domains are required for optimal E2A-dependent transcription. Surprisingly, however, neither activation domain is required for E2A to rescue B lymphopoiesis from E2A(-/-) hemopoietic progenitors, although the N terminus of E2A, which harbors some transcription capacity, is required. Therefore, the E protein activation domains function redundantly in promoting B cell development. In contrast, the N-terminal activation domain, AD1, is required for a newly described ability of E2A to suppress macrophage development in vitro. Our findings demonstrate distinct functionalities for the E protein activation domains in B lymphocytes and macrophages.

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Citations

Sep 14, 2012·Blood·Christopher M DenisSteven P Smith
Sep 6, 2012·Molecular and Cellular Biology·Ioanna ZarifiChristos Delidakis
Oct 26, 2010·Immunological Reviews·Renée F de Pooter, Barbara L Kee
Sep 26, 2013·Nucleic Acids Research·Chien-Hung GowJinsong Zhang
Jan 30, 2014·Annual Review of Immunology·Ellen V Rothenberg
Mar 5, 2016·BioMed Research International·Jason H RogersRichard Dahl
Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nikola SekulovskiJames A MacLean
May 4, 2011·Molecular and Cellular Biology·Aubrey HuntScott W Hiebert
Jul 18, 2021·Journal of Biological Research·Jia-Jie LiangJiang-Feng Wu
Jul 23, 2013·Genes & Development·Wei-Yi ChenRobert G Roeder

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